Evidence map›Paper›PMID 41317665›Full record

ArticleEBioMedicine2025

Early lymph node T follicular helper cell signalling hub drives influenza vaccine response in an ancestrally diverse cohort.

Jacqueline H Y Siu, Sofia Coelho, Aime Palomeras, Sandra Belij-Rammerstorfer, Ninisha Barman, Chloe H Lee, Tamara Ströbel, Christopher J Thorpe, Charandeep Kaur, Tom Cole and 21 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EBioMedicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Multimodal analysis definesbioRxiv : the preprint server for biology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

Jacqueline H Y SiuKennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK; Centre for Medicines Discovery and Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Sofia CoelhoNIHR Imperial Clinical Research Facility, Hammersmith Hospital, London, UK.
Aime PalomerasNIHR Imperial Clinical Research Facility, Hammersmith Hospital, London, UK.
Sandra Belij-RammerstorferOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Ninisha BarmanOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Chloe H LeeMRC Translational Immune Discovery Unit, Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
Tamara StröbelKennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.
Christopher J ThorpeEuropean Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, UK.
Charandeep KaurOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Tom ColeNIHR Imperial Clinical Research Facility, Hammersmith Hospital, London, UK.
Nico RemmertKennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.
Jamie FowlerOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Sam PledgerKennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.
Kyla B DooleyCentre for Medicines Discovery and Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK; Pandemic Sciences Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Terrence ChanOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Katja HöschlerRespiratory Virus Unit, UK Health Security Agency, UK.
Maria ZambonRespiratory Virus Unit, UK Health Security Agency, UK.
Daniel OpokaMedical Research Council/Uganda Virus Research Institute, and London School of Hygiene and Tropical Medicine, Uganda Research Unit, Entebbe, Uganda.
Tamas SzommerCentre for Medicines Discovery and Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Seung J KimOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Vinod KumarKennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.
Samantha VanderslottOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
Pontiano KaleebuMedical Research Council/Uganda Virus Research Institute, and London School of Hygiene and Tropical Medicine, Uganda Research Unit, Entebbe, Uganda.
Anita MilicicThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Donald B PalmerDepartment of Comparative Biomedical Sciences, Royal Veterinary College, London, UK.
Teresa LambeOxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK; Pandemic Sciences Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Brian D MarsdenCentre for Medicines Discovery and Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Hashem KoohyMRC Translational Immune Discovery Unit, Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
Mark ColesKennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.
Calliope A DendrouKennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK; Centre for Medicines Discovery and Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK; NIHR Oxford Biomedical Research Centre, Oxford, UK. Electronic address: calliope.dendrou@kennedy.ox.ac.uk.
Katrina M PollockNIHR Imperial Clinical Research Facility, Hammersmith Hospital, London, UK; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK; NIHR Oxford Biomedical Research Centre, Oxford, UK. Electronic address: katrina.pollock@paediatrics.ox.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEarly in vivo dynamics of human immune-cell activation across regionally activated lymphoid tissue sites upon immunisation are poorly characterised in ancestrally-diverse individuals with consequences for pandemic preparedness.

methodsIn this experimental medicine study, draining and non-draining lymph nodes (dLNs and ndLNs) were studied by ultrasound (US)-guided fine-needle aspiration (FNA) in 13 adults aged 18-55 years with African and Asian ancestry, before and after receiving adjuvanted seasonal influenza vaccine (aQIV). A multi-modal investigation of ultrasound data, genotyping, systems serology, and single-cell multi-omics was undertaken.

findingsHLA subtypes reflected self-declared ethnicity and included understudied alleles. Draining but not ndLNs rapidly increased in size post-vaccination, by day 3, with distinct cellular dynamics culminating in a cross-protective serological response. Dissecting LN cellular diversity into 42 lymphoid and non-lymphoid cell states, early post-vaccination cell abundance changes were observed across all LNs, but dLNs were characterised by CD4

interpretationEarly CD4

fundingThe study was funded by the Silicon Valley Community Foundation with a Chan Zuckerberg Initiative donation. The funder had no role in the study design, data analysis or decision to publish. The funder provided infrastructure support for the posting of the dataset with CELLxGENE.

Indexed as

Influenza, HumanInfluenza VaccinesLymph NodesSignal TransductionT Follicular Helper CellsAdolescentAdultFemaleHumansMaleMiddle AgedVaccinationYoung AdultInfluenza VaccinesAncestral diversityHuman lymph node fine needle aspiratesInfluenzaSingle-cell multi-omicsT follicular helper cellsVaccination

Identifiers

PMID41317665
PMCPMC12703867

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.