Evidence map›Paper›PMID 41317219›Full record

ArticleEsophagus : official journal of the Japan Esophageal Society2026

AI-driven spatial mapping of myxoid stroma and tumor-infiltrating lymphocytes in esophageal squamous cell carcinoma.

Keita Kouzu, Hironori Tsujimoto, Ines P Nearchou, Takahiro Einama, Yoshiki Kajiwara, Takanori Watanabe, Makiko Koga, Tomohiro Tsuru, Hiroyuki Horiguchi, Yoshihisa Yaguchi and 3 more

Abstract read
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Article in Esophagus : official journal of the Japan Esophageal Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Keita KouzuDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Hironori TsujimotoDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan. tsujihi@ndmc.ac.jp.ORCID 0000-0002-2808-4723
Ines P NearchouIndica Labs, LCC., 8700 Education Pl NW, Bldg. B, Albuquerque, NM, 87114, USA.
Takahiro EinamaDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Yoshiki KajiwaraDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Takanori WatanabeDepartment of Basic Pathology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, Japan.
Makiko KogaDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Tomohiro TsuruDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Hiroyuki HoriguchiDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Yoshihisa YaguchiDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Yoji KishiDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.
Hitoshi TsudaPathology Center and Department of Pathology, Chiba Medical Center, 1-7-1 Minami-cho, Chuo-ku, Chiba, 260-0842, Japan.
Hideki UenoDepartment of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-0042, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMyxoid stroma in the tumor stroma has been suggested to play a key role in cancer progression; however, its underlying mechanisms remain unclear. This study aimed to examine the relationship between myxoid stroma, identified in hematoxylin and eosin (HE)-stained tissue, and tumor-infiltrating lymphocytes (TILs), assessed through immunohistochemical staining. Automated image analysis was employed to evaluate these features, and their clinical significance in esophageal squamous cell carcinoma (ESCC) was investigated.

methodsA total of 139 ESCC patients who underwent esophagectomy were included. From each patient, three serial sections from paraffin blocks were prepared and stained with CD3, HE, and CD8, respectively. An algorithm for identifying myxoid stroma was applied to HE slides, and the resulting data were mapped onto the corresponding CD3 and CD8 slides to analyze the spatial relationship between these pathological findings.

resultsThe myxoid-high group, comprising 76 cases, had significantly deeper tumor depth compared to the myxoid-low group, which included 63 cases (p < 0.05). CD3

conclusionsIn conclusion, this study demonstrated that myxoid stroma in the invasive front of ESCC is closely associated with the density of CD3

Indexed as

Esophageal NeoplasmsEsophageal Squamous Cell CarcinomaLymphocytes, Tumor-InfiltratingAgedCD3 ComplexEsophagectomyFemaleHumansImmunohistochemistryMaleMiddle AgedStromal CellsCD3 ComplexDeep-learningDesmoplastic reactionDigital pathologyEsophageal cancerTumor infiltrating lymphocyte

Identifiers

PMID41317219

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.