Evidence map›Paper›PMID 41317171›Full record

ArticleMikrochimica acta2025

Development of surface-enhanced Raman scattering nanoprobes for the simultaneous multiplex detection of characteristic DNA sequences: case studies of pathogenic Vibrio parahaemolyticus and Apolipoprotein E gene polymorphisms.

Chih-Hsien Wang, Yu-Chen Chou, Hsin-Yun Li, Yen-Ta Tseng, Yu Ling Sun, Pei-Chen Jen, Shau-Chun Wang, Lai-Kwan Chau, Chung Lun Lu, Yen-Ling Chen

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Chih-Hsien Wang *Center for Nano Bio-Detection, National Chung Cheng University, Chiayi, 621301, Taiwan.
Yu-Chen Chou *Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, 621301, Taiwan.
Hsin-Yun Li *Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, 621301, Taiwan.
Yen-Ta TsengCenter for Nano Bio-Detection, National Chung Cheng University, Chiayi, 621301, Taiwan.
Yu Ling SunAquatic Technology Research Center, Agricultural Technology Research Institute, Hsinchu, 300110, Taiwan.
Pei-Chen JenDepartment of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, 621301, Taiwan.
Shau-Chun WangCenter for Nano Bio-Detection, National Chung Cheng University, Chiayi, 621301, Taiwan.
Lai-Kwan ChauCenter for Nano Bio-Detection, National Chung Cheng University, Chiayi, 621301, Taiwan. chelkc@ccu.edu.tw.
Chung Lun LuAquatic Technology Research Center, Agricultural Technology Research Institute, Hsinchu, 300110, Taiwan. 1041002@mail.atri.org.tw.
Yen-Ling ChenCenter for Nano Bio-Detection, National Chung Cheng University, Chiayi, 621301, Taiwan. yelichen@ccu.edu.tw.

Funding

National Science and Technology Council of Taiwan MOST 109-2123-M-194-001
6 · The paper itself

Abstract

Relying on a single biomarker in biomedical analysis is often insufficient for accurate disease or pathogen determination. A recent trend is using simultaneous multiplex detection of multiple biomarkers to improve diagnostic accuracy and throughput. To enable multiplex detection, we developed a series of surface-enhanced Raman scattering (SERS) nanoprobes, referred to as nanoaggregate-embedded beads (NAEBs). These NAEBs were synthesized using three distinct Raman reporter molecules: Safranin O, ethyl violet, and cresyl violet acetate. By integrating the NAEBs with magnetic nanoparticles and a simple capillary magnetofluidic device, we developed a rapid and simultaneous multiplex detection platform for genetic analysis of an aquacultural pathogen Vibrio parahaemolyticus (VP) for pirA, pirB, and ompA and genotyping of Alzheimer's disease's risk factor biomarker Apoliproprotein E (ApoE). For VP detection, a limit of detection (LOD) as low as ~ 10

Indexed as

Apolipoproteins ESpectrum Analysis, RamanVibrio parahaemolyticusHumansLimit of DetectionPolymorphism, Single NucleotideApolipoproteins EApoE geneIron oxide nanoparticlesMultiplex detectionNanoaggregate-embedded beadsSingle nucleotide polymorphismsSurface enhanced Raman scattering (SERS)Vibrio parahaemolyticus

Identifiers

PMID41317171

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.