ArticleEquine veterinary journal2026
Enhanced detection of equine strongyles: Insights from morphological and nemabiome metabarcoding approaches in northern Iran.
Article in Equine veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Enhanced detection of equine strongyles: Insights from morphological and nemabiome metabarcoding approaches in northern Iran.Equine veterinary journal · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundStrongyles pose significant health concerns for equids globally. Strongyles, comprising over 60 species, can lead to severe morbidity and mortality, with Strongylus vulgaris posing higher risks due to its migratory behaviour. Routine diagnostic methods, such as faecal egg counts, lack species-level resolution, while traditional morphological techniques require advanced expertise. DNA metabarcoding offers a high-throughput alternative.
objectivesTo characterise the diversity of strongyles infecting horses in northern Iran and evaluate how age, sex, diagnostic methods and host population influence community composition. STUDY
designCross-sectional.
methodsStrongyle communities were studied across four locations. At two farms, subsets of horses were analysed either by morphological identification of adult worms or by ITS2 metabarcoding of larval cultures. Morphological identification was performed on 1476 adult worms recovered from 23 horses at two farms (Rezvanshahr and Gisum). In parallel, ITS2 nemabiome metabarcoding was applied to pools of ~2500 L3 larvae from faeces of 25 untreated horses. Community composition was analysed using dissimilarity indices (Jaccard, Bray-Curtis), PERMANOVA and generalised linear models to assess the effects of farm, method, age and sex.
resultsThirty-three species were detected across both methods. DNA metabarcoding identified more species and 11 species were recorded in Iran for the first time. Strongyle community composition varied significantly among locations, including between resident and non-resident horses at the riding club, and between diagnostic methods. Neither horse age nor sex explained variation. S. vulgaris was prevalent across the majority of locations, potentially due to inconsistent treatment. MAIN LIMITATIONS: Morphological and nemabiome identifications were conducted on different subsets of horses in the same location, precluding direct within-individual comparisons. The study relied on owner-reported information about horse characteristics and management practices.
conclusionThese findings provide new insights into strongyle diversity in northern Iran and highlight the value of molecular diagnostics for equine parasite surveillance and control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.