Evidence map›Paper›PMID 41316426›Full record

ArticleVeterinary research2025

Pathogenic and transcriptomic differences among porcine reproductive and respiratory syndrome viruses from distinct lineages in piglets.

Linxing Tian, Xingran Wang, Zhenxiang Rong, Mengxin Zhang, Yawei Sun, Ruiqu Zhou, Jiahui Ma, Chao Zhang, Shudan Liu, Nan Cao and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Linxing TianNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Xingran WangNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zhenxiang RongNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Mengxin ZhangNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Yawei SunNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Ruiqu ZhouNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Jiahui MaNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Chao ZhangNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Shudan LiuNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Nan CaoNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zihui HuNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Jiyuan LuoNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Xiangmin LiNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. lilixiangmin@mail.hzau.edu.cn.
Ping QianNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. qianp@mail.hzau.edu.cn.

Funding

National Key Laboratory of Agricultural Microbiology AML2023B11National Key Research and Development Program of China 2022YFD1800800National Key Research and Development Program of China 2024YFD1800505
6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly recombinogenic pathogen that threatens global swine production. Multiple PRRSV lineages co-circulate in China, with lineage 1.8 being the predominant epidemic strain. However, the pathogenic characteristics and differences among strains from distinct lineages remain insufficiently studied. In this study, three novel recombinant PRRSV strains (GX-2428, GX-3264, and GX-5430) were isolated in Guangxi, China. Phylogenetic analysis of the ORF5 gene classified the three strains into lineage 3 (QYYZ-like), lineage 1.8 (NADC30-like), and lineage 1.5 (NADC34-like), respectively. Pathogenicity tests in piglets demonstrated that, compared with the control group, both GX-2428 and GX-3264 induced significant fever, whereas GX-5430 caused only a transient and milder febrile response. Infected piglets exhibited elevated levels of pro-inflammatory (IL-1β and TNF-α) and immunomodulatory cytokines (IL-4 and IL-10) compared to the control group. Postmortem analysis revealed that although viral shedding had ceased, high viral loads persisted in the lungs, tonsils, and lymph nodes of the infected piglets. Transcriptomic analysis of piglet lung tissues revealed that GX-5430 infection predominantly enriched pathways related to cellular transformation, signal transduction, and metabolic reprogramming. However, infections with GX-2428 or GX-3264 were significantly enriched in immune-related pathways, thereby inducing stronger immune activation and inflammatory responses. In conclusion, these findings highlight the recombination characteristics and lineage-specific pathogenic mechanisms of PRRSV, providing novel insights for the development of future prevention and control strategies.

Indexed as

Porcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusTranscriptomeAnimalsChinaCytokinesPhylogenySwineCytokineshost responsespathogenicityPorcine reproductive and respiratory syndrome virusrecombinationtranscriptomic analysis

Identifiers

PMID41316426
PMCPMC12664146

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.