ArticleStem cell research & therapy2025
Tissue regenerative potential of human dental pulp mesenchymal stem cell conditioned medium in wound healing of the medicinal leech.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Secreted Peptidome Profiling of Human Dental Pulp Stem Cells-Conditioned Medium During Odontogenic Differentiation.International dental journal · 2026Article
- Evaluating 3D-Patch Efficacy in Wound Healing Using the Medicinal LeechNanomaterials (Basel, Switzerland) · 2026Article
- Nanostructured Scaffold, Combined with Human Dental Pulp Stem Cell Secretome, Induces Vascularization in Medicinal Leech Model.Micromachines · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
backgroundAs life expectancy increases, so does the incidence of age-related disorders, including vascular diseases and chronic wounds. These conditions are often associated with impaired angiogenesis and tissue repair, posing significant clinical challenges due to prolonged healing times. Recent studies have highlighted the tissue regenerative potential of the human Mesenchymal Stem Cell-Conditioned Medium (MSC-CM). Building on this, we investigated the ability of CM derived from human Dental Pulp Stem Cells (hDPSC-CM) to support angiogenesis and tissue repair in a surgically injured medicinal leech model. The medicinal leech Hirudo verbana offers a valuable in vivo tool for studying wound healing and regeneration, due to its well-characterized anatomy, regenerative capabilities, and the evolutionary conservation of key immune and vascular pathways.
methodsAdult leeches were surgically injured and treated with either FBS-free medium (CTRL) or hDPSC-CM. Animals were sacrificed at 48 h, 72 h, and 1 week post-treatment to evaluate tissue regeneration across experimental groups. Morphological, immunohistochemical, and molecular analyses were conducted.
resultshDPSC-CM significantly accelerated wound healing, promoting extracellular matrix remodelling and muscle regeneration. The expression of key markers acid phosphatase, CD31, VEGF, Desmin, AIF-1, and COL Iα1, along with vessel counts, confirmed the immunomodulatory activity (38.5% overall decrease in macrophage number), enhanced angiogenesis (64.2% overall increase in CD31 expression), and improved muscle regeneration (222.5% overall increase in Desmin expression) induced by hDPSC-CM in the injured area.
conclusionsOur findings support the therapeutic potential of hDPSC-CM as a novel, cell-free strategy for enhancing wound healing and managing chronic conditions related to impaired tissue repair.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.