ArticleBMC biotechnology2025
Immobilization of bacterial spores using eggshells nanoparticles and their effective role in concrete bio-healing process: novel approach.
Article in BMC biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Microbial induced calcium carbonate precipitation under extreme environmental conditions: impacts on biomineralization process and mechanical performance of concrete.Frontiers in bioengineering and biotechnology · 2026Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrobially induced calcium carbonate precipitation (MICP) has gained increasing attention as a sustainable bioengineering strategy for improving the strength and bio-healing capacity of concrete. Through biologically mediated CaCO₃ formation, microorganisms can bridge cracks and enhance material cohesion. A persistent challenge, however, lies in maintaining bacterial viability under the mechanically harsh conditions of cementitious systems. To overcome this issue, the present study introduces eggshell-derived nanoparticles (EShN) as an innovative, low-cost carrier for bacterial spores, aiming to improve their protection.
methodsThe size, chemical composition of the EShN, and the spores’ immobilization process were examined using methods such as X-ray diffraction (XRD), scanning electron microscopy (SEM-EDX), and transmission electron microscopy (TEM). The biohealing of concrete and its strengths were investigated.
resultsSEM imaging revealed efficient immobilization of Bacillus spores onto the nanoparticle surfaces, while EDX analysis verified calcium carbonate as the main mineral formed within healed cracks. Concrete samples incorporating immobilized bacterial spores exhibited remarkable enhancements in mechanical performance, showing increases of approximately 45, 49.5, and 47.5% in compressive, tensile, and flexural strengths, respectively, compared with negative control.
conclusionsThis research demonstrates that eggshell nanoparticles serve as an effective and sustainable medium for bacterial immobilization, significantly improving both the healing efficiency and mechanical properties of bio-concrete. Overall, this approach provides a promising pathway toward the development of resilient, eco-friendly, and biorepairing construction materials.
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