Evidence map›Paper›PMID 41315643›Full record

ArticleScientific reports2025

Dynamic assessment of a humanized bone tumour microenvironment reveals insights into osteosarcoma primary tumour remodelling and lung metastases.

Jonathan Gospos, Markus Laubach, Flavia Medeiros Savi, Akhilandeshwari Ravichandran, Julian Bauer, Oliver Friedrich, Boris M Holzapfel, Ferdinand Wagner, Tomoji Mashimo, Siamak Saifzadeh and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jonathan GosposMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia.ORCID 0009-0005-3836-9852
Markus LaubachCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, 4000, Australia.ORCID 0000-0002-3451-1445
Flavia Medeiros SaviMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia.ORCID 0000-0003-0067-8308
Akhilandeshwari RavichandranCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD, 4000, Australia.ORCID 0000-0003-4807-1150
Julian BauerMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia.ORCID 0000-0002-2149-1094
Oliver FriedrichMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia.ORCID 0000-0003-2238-2049
Boris M HolzapfelDepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig Maximilian University (LMU) Munich, Munich, Germany.ORCID 0000-0002-3989-2531
Ferdinand WagnerDepartment of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig Maximilian University (LMU) Munich, Munich, Germany.ORCID 0000-0001-8565-8722
Tomoji MashimoDivision of Animal Genetics, Laboratory Animal Research Centre, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.ORCID 0000-0001-7543-7301
Siamak SaifzadehAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of Technology, Brisbane, QLD, 4000, Australia.ORCID 0000-0002-0295-7477
Dietmar W HutmacherMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia.ORCID 0000-0001-5678-2134
Jacqui A McGovernMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia. Jacqui.mcgovern@qut.edu.au.ORCID 0000-0002-4993-6745

Funding

Alexander von Humboldt-Stiftung Feodor Lynen Research FellowshipCancer Australia 1181919Deutscher Akademischer Austauschdienst 132446026Deutscher Akademischer Austauschdienst 57445433Deutscher Akademischer Austauschdienst 57654775National Health and Medical Research Council 2036703
6 · The paper itself

Abstract

Osteosarcoma (OS) is the most common primary bone cancer in children and adolescents, with poor prognosis linked to metastasis and recurrence. Preclinical models that replicate human disease progression and the tumour microenvironment (TME) are critical for advancing therapies. We developed a novel orthotopic and humanised bone tumour microenvironment model in highly immunocompromised Il2rg and Rag2 double knockout rats. A humanised bone niche (HN) was established by implanting orthotopic humanised bone constructs (ohTEBCs) around the rat femur, followed by SaOS-2-luc cell injection. Longitudinal µCT and bioluminescent imaging tracked HN formation, tumour growth, and metastasis. Histological and immunohistochemical analyses revealed pathological mineralisation, extensive collagen deposition, tumour-specific ECM production, and neovascularisation within primary tumours and lung metastases. This model supports consistent engraftment of SaOS-2-luc tumours with robust metastatic spread, recapitulating key human OS features. It offers a translatable preclinical platform for studying OS progression and testing chemotherapy and surgical interventions.

Indexed as

Bone NeoplasmsLung NeoplasmsOsteosarcomaTumor MicroenvironmentAnimalsCell Line, TumorDisease Models, AnimalHumansRats

Identifiers

PMID41315643
PMCPMC12753712

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.