background

methodsThe present study investigates the interactions of plant secondary metabolites tables on Mex B efflux protein. It identifies lead molecules for developing adjuvants against efflux-mediated multidrug resistance in P. aeruginosa infections, enhances antibiotic activity against MDR pathogens, and evaluates the MIC value of the test plant compound (Bisabolol) and the resistant antibiotic (Meropenem).

resultsAmong plant compounds, α-Bisabolol, myricetin, capsaicin, equenin, aloe-emodin, terpinene, fisetin, taxifolin, catechin, and galangin showed G-Scorehigher than -7 kcal/mol, and interact with active amino acids Mex B efflux protein which may affect the efflux transport of drug and enhance the antibiotic activity against MDR infection. According to docking experiments, α-bisabolol has a higher affinity energy to the MexB protein than Meropenem. Furthermore, α-bisabolol binds to the MexB binding site hydrophobic trap region of MexB, which may cause a conformational change in the transporter's pumping process, thereby affecting antibiotic efflux inhibition. The MICs against Meropenem Pseudomonas Aeruginosa were 12.5 μg/ml for antibiotic Meropenem and 6.24 μg/ml for the combination.

conclusionThe study concluded that these plant secondary metabolite compounds could be used to develop adjuvant along with antibiotics to increase their activity against MexAB-OprM efflux-mediated multidrug-resisting infections. It was determined that α-bisabolol may have the potential to boost antibacterial activity when combined with antibiotics, as well as being a strong candidate for an efflux pump inhibitor. This is the first inclusion of the properties of a natural plant phytochemical, Bisabolol, utilized in combination with commercial resistant antibiotic Meropenem to enhance its activity against MDR pneumonia infection caused by Pseudomonas Aeruginosa.