ArticleArchives of microbiology2025
Baculovirus expression and purification of nucleocapsid protein of canine distemper virus for its evaluation as a diagnostic antigen.
Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
Canine distemper is a highly contagious disease of domestic dogs and various species of wild animals, and is associated with high mortality. It is caused by canine morbillivirus (formerly known as CDV or canine distemper virus), a member of the Morbillivirus genus under Paramyxoviridae family. The disease is endemic in many parts of the world, including India. For effective control of the disease, reliable diagnostic tools are of utmost importance. The circulating CDV strains in India belong to a novel "India1/Asia-5" lineage, highlighting the need for the development of new diagnostic tools. The nucleocapsid (N) protein is the major structural protein and one of the most immunogenic proteins of the virus; thus, it has been the preferred diagnostic target. Therefore, the present study was aimed at expressing the nucleocapsid protein of CDV using the baculovirus expression system in insect cells and its successful purification using affinity column chromatography. Expression and specificity of the recombinant N protein were confirmed using SDS-PAGE and Western blot analysis, revealing a band of approximately 58 kDa. The purified protein also reacted with CDV polyclonal serum, suggesting that the N protein was expressed in an immunoreactive form. Among the panel of monoclonal antibodies generated against CDV, only CDV-2F8 exhibited specific reactivity with the recombinant N protein, indicating the preservation of at least one linear epitope. The diagnostic suitability of the expressed protein was further confirmed by using it as a coating antigen in an indirect ELISA, which clearly distinguished between CDV-positive and negative dog serum samples. The recombinant N protein developed in the present study can facilitate large-scale field application of recombinant protein-based diagnostics for CDV, as it eliminates the need for live virus antigen in routine serodiagnostic assays.
Indexed as
Identifiers
41313480What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.