Proposal of metagenomic-origin LRA-5 as a precursor of active β-lactamases through Tyr69Gln and Val166Glu amino acid substitutions: a functional and structural analysis.
Gabriela D'Amico González, María Margarita Rodríguez, Pedro Penzotti, Florencia Brunetti, Barbara Ghiglione, Luke A Moe, Daniela Centrón, Gabriel Gutkind, Lin Gao, Shozeb Haider and 3 more
Article in Antimicrobial agents and chemotherapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
13 authors.
Gabriela D'Amico GonzálezFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Buenos Aires, Argentina.
María Margarita RodríguezFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Buenos Aires, Argentina.
Pedro PenzottiFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Buenos Aires, Argentina.
Florencia BrunettiFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Buenos Aires, Argentina.
Barbara GhiglioneFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Buenos Aires, Argentina.
Luke A MoeUniversity of Kentucky, Plant and Soil Sciences, Lexington, Kentucky, USA.ORCID 0000-0001-6864-4660
Daniela CentrónConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Gabriel GutkindFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Buenos Aires, Argentina.ORCID 0000-0003-1999-9785
Lin GaoUCL School of Pharmacy, London, United Kingdom.
Rachel A PowersDepartment of Chemistry, Grand Valley State University, Allendale, Michigan, USA.ORCID 0000-0002-9968-8284
Sebastián Klinke *Fundación Instituto Leloir, IIBBA-CONICET, and Plataforma Argentina de Biología Estructural y Metabolómica PLABEM, Buenos Aires, Argentina.ORCID 0000-0002-8777-0870
Pablo Power *Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Buenos Aires, Argentina.ORCID 0000-0002-7051-9954
Funding
Characterizing novel Beta-lactamases from metagenomic samples: insights into the evolution of antimicrobial resistance from the environment towards human-associated pathogensR01AI160371 · NIAID · UBATEC S.A. · PI Pablo Power · 2022 to 2026
$630k
Agencia Nacional de Promoción Científica y Tecnológica PICT-2021-I-A-0771Consejo Nacional de Investigaciones Científicas y Técnicas 11220200100191CONIAID NIH HHS R01 AI160371NIH HHS R01AI160371Universidad de Buenos Aires 2023
6 · The paper itself
Abstract
Wild-type LRA-5, recovered from Alaskan soil samples, shares no more than 33% amino acid sequence identity with enzymes from pathogens like PER β-lactamases. Recombinant
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Proposal of metagenomic-origin LRA-5 as a precursor of active β-lactamases through Tyr69Gln and Val166Glu amino acid substitutions: a functional and structural analysis. · full record | OpenQuestion