Evidence map›Paper›PMID 41311941›Full record

ArticleJACS Au2025

Nick-and-Digest Strategy for Programmable Circular ssDNA Production and Scalable DNA Origami Assembly.

Jingyi Ye, Ao Liu, Hui Lv, Chenyun Sun, Fei Wang, Huan Zhang, Honglu Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JACS Au, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jingyi YeSchool of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 511442, China.
Ao LiuSchool of Automation and Intelligent Sensing, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Hui LvInstitute of Materiobiology, College of Sciences, Shanghai University, Shanghai 200444, China.
Chenyun SunSchool of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, New Cornerstone Science Laboratory, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
Fei WangSchool of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, New Cornerstone Science Laboratory, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
Huan ZhangSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID https://orcid.org/0000-0002-1096-4255
Honglu ZhangSchool of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 511442, China.ORCID https://orcid.org/0000-0003-3930-6739

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA origami enables the precise self-assembly of complex nanostructures with applications in drug delivery, biosensing, and nanoelectronics. However, the scalability of DNA origami is constrained by the limited length of the available single-stranded DNA (ssDNA) scaffolds. Here, we present a sequence-flexible "nick-and-digest" strategy to generate long circular ssDNA (cssDNA) scaffolds of customizable lengths directly from plasmid DNA. By combining Cas9 (D10A) nickase (Cas9n) with T7 exonuclease (T7 Exo), we generate high-purity cssDNA scaffolds of around 7,000 and 15,000 nucleotides (7k-nt and 15k-nt) with minimal sequence dependence. These extended scaffolds enable the one-pot folding of large-scale origami structures (147 × 107 nm) that double the surface area of conventional 7 kb designs. We optimize the denaturation temperature, annealing procedure, and staple-to-scaffold ratios to improve the folding efficiency while minimizing thermal damage. Compared to a two-step dimerization approach, the one-pot assembly achieves higher yield, fewer structural defects, and greater mechanical stability, as confirmed by atomic force microscopy (AFM) and coarse-grained molecular dynamics (one-pot: -15.19  ±  0.014

Indexed as

Atomic force microscopy (AFM)Circular single-stranded DNA (cssDNA)DNA molecular dynamicsDNA origamiOne-pot assembly

Identifiers

PMID41311941
PMCPMC12648328

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.