Evidence map›Paper›PMID 41311496›Full record

ArticleFrontiers in microbiology2025

Recombinant expression and functional characterization of influenza A virus neuraminidase in a mammalian cell system.

Ailing Huang, Yulong Liu, Yanbai Li, Zhe Yin, Shanshan Huo, Juan Wang, Zhenlin Yang, Tianlei Ying, Fei Yu

Abstract read
PubMed Full text at PMC Publisher
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Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ailing HuangHebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, College of Life Sciences, Hebei Agricultural University, Hebei, China.
Yulong LiuKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Yanbai LiHebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, College of Life Sciences, Hebei Agricultural University, Hebei, China.
Zhe YinHebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, College of Life Sciences, Hebei Agricultural University, Hebei, China.
Shanshan HuoHebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, College of Life Sciences, Hebei Agricultural University, Hebei, China.
Juan WangHebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, College of Life Sciences, Hebei Agricultural University, Hebei, China.
Zhenlin YangShanghai Engineering Research Center for Synthetic Immunology, Shanghai, China.
Tianlei YingKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Fei YuHebei Key Laboratory of Analysis and Control of Zoonotic Pathogenic Microorganism, College of Life Sciences, Hebei Agricultural University, Hebei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The soluble and functional expression of influenza A virus neuraminidase (NA) remains a major challenge due to its membrane-associated nature and structural complexity. In this study, we established a mammalian expression strategy that enables the production of correctly folded, enzymatically active NA proteins across multiple influenza A subtypes. Through systematic N-terminal deletion mapping, we identified a conserved structural boundary within the transmembrane-stalk region that critically determines NA solubility. Truncation of approximately 65-80 amino acids, combined with enforced tetramerization

Indexed as

broad-spectrum vaccineinfluenza A virusmammalian expressionneuraminidasetetrameric assembly

Identifiers

PMID41311496
PMCPMC12647020

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.