ArticlePlant biotechnology journal2026
Rapid Genetic Stabilisation of Transgenic and Edited Wheat Through Transformation of Immature Haploid Embryos.
Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Metabolic engineering of cereal lipids: from omega-3 fatty acids to wax esters and pheromones.Advanced biotechnology · 2026Review
- The Auxin Response FactorPlants (Basel, Switzerland) · 2026Article
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Authors and funding
13 authors.
Funding
Abstract
Wheat is one of the globally important food crops, but its genetically modified application lags behind other staple crops due to its complex genome and low transformation efficiency. Normally, traditional transgenic and gene-edited wheat requires multiple selfing generations for obtaining homozygous lines, which delayed the development of genetically modified wheat varieties. In this study, we crossed a haploid inducer line with purple embryos (HIPE) as a male parent with a wheat cultivar Fielder, and then chose the haploid immature embryos without purple colour to be transformed with the Agrobacterium cells carrying the expression vectors with GUS, RUBY, and Cas9 and sgRNAs for targeting TaWaxy for generating both transgenic and gene editing wheat plants. Consequently, 139 haploid transgenic wheat plants were obtained with transformation efficiencies ranging from 66.7% to 78.4% according to molecular analysis, histochemical staining assay and colour observation. After chromosome doubling treatment to the haploid transgenic plantlets, homozygous transgenic and gene-edited wheat lines were produced in T
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