Evidence map›Paper›PMID 41311041›Full record

ArticleParasites, hosts and diseases2025

Vaccinia virus expressing MIC8 and AMA1 provides protection against Toxoplasma gondii ME49 infection.

Hae-Ji Kang, Yan Jin, Zhao-Shou Yang, Md Atique Ahmed, Fu-Shi Quan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites, hosts and diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Veterinary sciences · 2026
    Review
  2. Protection AgainstPharmaceutics · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Hae-Ji KangDepartment of Microbiology, College of Medicine, Dongguk University, Gyeongju 38066, Korea.
Yan JinDepartment of Microbiology, College of Medicine, Dongguk University, Gyeongju 38066, Korea.
Zhao-Shou YangThe First Affiliated Hospital, The First Clinical Medicine School of Guangdong Pharmaceutical University, Guangdong Pharmaceutical University, Guangzhou 510080, China.
Md Atique AhmedICMR-Regional Medical Research Centre, NER, Dibrugarh 786010, India.
Fu-Shi QuanDepartment of Medical Zoology, College of Medicine, Kyung Hee University, Seoul 02447, Korea.

Funding

Ministry of EducationNational Research Foundation of Korea 2018-R1A6A1A03025124
6 · The paper itself

Abstract

Toxoplasma gondii is an intracellular protozoan parasite capable of causing chronic infection by forming persistent cysts in the brain. Despite its global burden, no approved vaccine exists. Virus-like particle vaccines expressing microneme protein 8 (MIC8) or apical membrane antigen 1 (AMA1) of T. gondii have previously shown efficacy. In this study, we generated recombinant vaccinia viruses (rVVs) expressing MIC8 and AMA1 and evaluated their efficacy against T. gondii ME49 infection. BALB/c mice were intramuscularly immunized with a combination of MIC8 and AMA1 rVVs and challenged orally with T. gondii ME49. Immunization with MIC8+AMA1 rVVs produced a significant increase in T. gondii-specific IgG. Splenocyte analysis revealed robust activation of CD4+ and CD8+ T cells, as well as expansion of memory B cells. The immunized group exhibited an 89.6% reduction in brain cyst count, with significantly improved survival compared to the control group. These findings demonstrate that combining the antigens MIC8 and AMA1 using a vaccinia virus platform can effectively promote both humoral and cellular immunity, supporting its potential as a vaccine strategy against T. gondii ME49.

Indexed as

Antigens, ProtozoanMembrane ProteinsProtozoan ProteinsProtozoan VaccinesToxoplasmaToxoplasmosis, AnimalVaccinia virusAnimalsAntibodies, ProtozoanFemaleImmunoglobulin GMiceMice, Inbred BALB CAMA1 protein, Toxoplasma gondiiAntibodies, ProtozoanAntigens, ProtozoanImmunoglobulin GMembrane ProteinsProtozoan ProteinsProtozoan VaccinesAMA1MIC8mouseprotective effectsrecombinant vaccinia virusToxoplasma gondii

Identifiers

PMID41311041
PMCPMC12661178

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.