Evidence map›Paper›PMID 41310911›Full record

ArticleJournal of biophotonics2026

Profiling Protein Citrullination in Extracellular Vesicles by Single-Molecule Detection Using Direct Stochastic Optical Reconstruction Microscopy.

Sarah R Needham, Benjamin M Davis, Pinar Uysal-Onganer, Daniel J Rolfe, Mariya Hristova, Igor Kraev, Jameel M Inal, Sigrun Lange

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biophotonics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sarah R NeedhamCentral Laser Facility, UKRI: Science & Technology Facilities Council, Rutherford Appleton Laboratory, Oxfordshire, UK.
Benjamin M DavisCentral Laser Facility, UKRI: Science & Technology Facilities Council, Rutherford Appleton Laboratory, Oxfordshire, UK.
Pinar Uysal-OnganerCancer Mechanisms and Biomarkers Research Group, School of Life Sciences, University of Westminster, London, UK.
Daniel J RolfeCentral Laser Facility, UKRI: Science & Technology Facilities Council, Rutherford Appleton Laboratory, Oxfordshire, UK.
Mariya HristovaDepartment of Neonatology, EGA Institute for Women's Health, University College London, London, UK.
Igor KraevElectron Microscopy Suite, Faculty of Science, Technology, Engineering and Mathematics, Open University, Milton Keynes, UK.
Jameel M InalCell Communication in Disease Pathology, School of Human Sciences, London Metropolitan University, London, UK.
Sigrun LangePathobiology and Extracellular Vesicles Research Group, School of Life Sciences, University of Westminster, London, UK.ORCID 0000-0002-7193-3102

Funding

UKRI through access to STFC's Central Laser Facility's Octopus Facility LSF 25130012
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are critical in cellular communication and pathological biomarkers. Post-translationally deiminated/citrullinated proteins are reported in EV cargoes by LC-MS/MS but it is unknown in which EV sub-types they are exported, as part of EVs' intraluminal cargo or on the EV surface. Here, dSTORM super-resolution microscopy is used to co-localise total citrullinated proteins (pan-Cit), and citrullinated histone H3 (CitH3) to EV subtypes of three cancer cell lines, captured by tetraspanin trio (TT) or phosphatidylserine (PS). Permeabilised and non-permeabilised EVs are analysed with a Bayesian framework using beta-distributed posteriors for binomial outcomes. Pan-Cit and CitH3 labelling is confirmed in EVs as intraluminal cargo and on the EV surface, with higher levels detected in the permeabilized EVs. Pan-Cit staining is higher in TT-bound EVs, but CitH3 staining higher in PS-bound EVs. This study expands the landscape of EV-associated post-translational modifications with translational potential for EV-citrullinome based liquid biopsy tools.

Indexed as

CitrullinationExtracellular VesiclesMicroscopySingle Molecule ImagingCell Line, TumorHistonesHumansStochastic ProcessesHistonesBayesian modelscitrullinated histone H3citrullination/deiminationdSTORMextracellular vesiclesliquid biopsysingle‐molecule detection

Identifiers

PMID41310911
PMCPMC12976978

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.