Evidence map›Paper›PMID 41310493›Full record

ArticleBMC infectious diseases2025

Comparison of different methods for the detection of HLA-B* 57:01 allele in people living with HIV in Eastern Uttar Pradesh, India.

Tulika Kumari Rai, Jaya Chakravarty, Kumari Priyanka, Abhilasha Gautam, Shweta Srivastva

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Tulika Kumari Rai *Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Jaya Chakravarty *Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India. tapadar@gmail.com.
Kumari PriyankaDepartment of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Abhilasha GautamDepartment of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Shweta SrivastvaDepartment of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.

Funding

Center of Excellence (COE) for HIV care , National Aids Control Organization (NACO) & Uttar Pradesh State AIDS control society (UPSACS) (Scheme No. 4080)
6 · The paper itself

Abstract

backgroundThe gold standard for detecting HLA-B*57:01 allele linked to Abacavir hypersensitivity is sequence-based typing (SBT), which is labour-intensive, not readily available and needs special expertise. HCP5 gene polymorphism is a surrogate marker for HLA-B*57:01 allele. Therefore, we compared, nested PCR for HLA-B*57:01 allele, HCP5 PCR with SBT method to develop a simple and cost-effective method for detection of HLA-B*57:01 allele.

methodWhole blood cells were collected from PLHIV and Buffy coat and DNA was isolated. Direct PCR for HLA-B*57 gene followed by nested PCR for the HLA-B* 57:01 allele and HCP5 gene PCR was done in all patients. The PCR results of both methods were confirmed by the SBT method.

resultAmong 366 samples, 25 (6.83%) were positive for HLA-B* 57:01 allele by nested PCR. HCP5 gene PCR was positive in 25 (6.83%) individuals who were also positive for the HLA-B*57:01 allele with nested PCR. A strong correlation was observed between the HLA-B*57:01 allele and HCP5 with negative and positive predictive values of 100% respectively in our population.

conclusionNested (two-step) and HCP5 gene PCR (single-step) are simple, rapid and cost-effective methods for HLA-B*57:01 allele detection and either can be adapted in the ART centre located in resource-poor settings.

Indexed as

HIV InfectionsHLA-B AntigensPolymerase Chain ReactionAbacavirAdultAllelesAnti-HIV AgentsCyclopropanesDideoxyadenosineDideoxynucleosidesFemaleHumansIndiaMaleMiddle AgedRNA, Long NoncodingAbacavirAnti-HIV AgentsCyclopropanesDideoxyadenosineDideoxynucleosidesHCP5 long noncoding RNA, humanHLA-B*57:01 antigenHLA-B57 antigenHLA-B AntigensRNA, Long NoncodingAbacavirHypersensitivity reactionLinkage disequilibrium (LD)People living with HIVPolymerase chain reaction

Identifiers

PMID41310493
PMCPMC12659622

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