ArticleWorld journal of microbiology & biotechnology2025
Development and application of a rapid dual RPA-LFS assay for simultaneous detection of Enterococcus faecalis and Enterococcus faecium.
Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Simultaneous point-of-care detection and virulence typing of Helicobacter pylori using multiplex recombinase polymerase amplification combined with lateral flow strip.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Establishment and clinical application of a rapid RPA-LFS assay for detectingFrontiers in cellular and infection microbiology · 2026Article
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Authors and funding
5 authors.
Funding
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Abstract
Enterococcus faecium and Enterococcus faecalis, ranked among the top three global nosocomial pathogens (WHO data), pose escalating threats due to multidrug resistance (particularly vancomycin-resistant strains, VRE) and foodborne transmission. Conventional detection relies on time-consuming culture-based typing (ISO 7899-2; 48-72 h) with > 12% false-negative rates, while molecular methods like qPCR require sophisticated instrumentation, limiting field deployment. This study developed a rapid dual recombinase polymerase amplification-lateral flow strip (RPA-LFS) assay for simultaneous detection of E. faecium and E. faecalis, addressing critical bottlenecks of prolonged turnaround time, species differentiation challenges, and equipment dependency. Species-specific primers and probes were designed targeting conserved genomic regions. Probes featured 5'-FITC/DIG reporter groups and amplification products incorporated 3'-biotin modifications, enabling THF cleavage-mediated signal release. Following optimization (37 °C, 20 min), results were visually interpreted via LFS within 10 min. Key findings include: Sensitivity: 10 CFU/mL (E. faecalis) and 10
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