Evidence map›Paper›PMID 41310205›Full record

ArticleParasitology research2025

Preliminary investigation of heat shock protein 90 gene diversity in Echinococcus granulosus sensu lato: a potential nuclear marker for species identification.

Zemzemi Lamia, Oudni-M'rad Myriam, Châabane-Banaoues Raja, Kamoun Ines, Gérald Umhang, Abdelghani Mohamed Hedi, Sayadi Taoufik, Messaoud Marwa, Sahnoun Lassaad, Franck Boué and 2 more

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Article in Parasitology research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Zemzemi Lamia *Faculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), University of Monastir, Monastir, LR12ES08, 5000, Tunisia.ORCID http://orcid.org/0009-0009-7869-5408
Oudni-M'rad Myriam *Faculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), University of Monastir, Monastir, LR12ES08, 5000, Tunisia.ORCID http://orcid.org/0000-0003-3344-9309
Châabane-Banaoues RajaFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), University of Monastir, Monastir, LR12ES08, 5000, Tunisia.ORCID http://orcid.org/0000-0002-3145-0165
Kamoun InesFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), University of Monastir, Monastir, LR12ES08, 5000, Tunisia.
Gérald UmhangNancy Laboratory for Rabies and Wildlife, Anses, Malzéville, France.ORCID http://orcid.org/0000-0001-6944-7474
Abdelghani Mohamed HediFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), University of Monastir, Monastir, LR12ES08, 5000, Tunisia.
Sayadi TaoufikCentral slaughterhouse of Sousse, Sousse, 4000, Tunisia.
Messaoud MarwaDepartment of pediatric surgery, University hospital F. Bourguiba, Monastir, 5000, Tunisia.
Sahnoun LassaadDepartment of pediatric surgery, University hospital F. Bourguiba, Monastir, 5000, Tunisia.
Franck BouéNancy Laboratory for Rabies and Wildlife, Anses, Malzéville, France.ORCID http://orcid.org/0000-0003-0957-1320
Babba HamoudaFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), University of Monastir, Monastir, LR12ES08, 5000, Tunisia.
M'rad SelimFaculty of Pharmacy, Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), University of Monastir, Monastir, LR12ES08, 5000, Tunisia. selim.mrad@gnet.tn.ORCID http://orcid.org/0000-0003-1298-3289

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus sensu lato (s.l.), remains a significant zoonotic parasitic infection worldwide. This study provides a preliminary investigation of the nuclear Hsp90 gene diversity within the E. granulosus s.l. complex to evaluate its potential use for species identification. Forty-nine DNA samples of the G1 genotype from human and animal CE cysts, two G3 genotype samples, one of Echinococcus ortleppi, four of Echinococcus canadensis (G7), and four samples of other Taenia species (Echinococcus multilocularis, Taenia hydatigena, Taenia pisiformis, and Taenia ovis) were analyzed. Four primer pairs were designed to amplify the Hsp90 gene, followed by PCR amplification, DNA sequencing, and phylogenetic analysis. Successful amplification and sequencing of nearly the entire Hsp90 gene revealed a single nucleotide polymorphism (SNP) at position 222 conserved across all genotypes. Notably, significant genetic variations were observed between E. ortleppi (G5 genotype) and E. canadensis (G7 genotype) compared to E. granulosus sensu stricto (G1 and G3 genotypes). Phylogenetic analysis confirmed clustering consistent with established taxonomic relationships, with G1 and G3 forming a cluster, and G5 and G7 forming a distinct group. The findings suggest that the nuclear Hsp90 gene could be used as an additional marker for species-level differentiation within the E. granulosus s.l. complex.

Indexed as

EchinococcosisEchinococcus granulosusGenetic VariationHSP90 Heat-Shock ProteinsAnimalsCluster AnalysisDNA, HelminthGenotypeHelminth ProteinsHumansPhylogenyPolymerase Chain ReactionPolymorphism, Single NucleotideSequence Analysis, DNADNA, HelminthHelminth ProteinsHSP90 Heat-Shock ProteinsCystic echinococcosisEchinococcus granulosus sensu latoGenetic diversityGenotypingHeat shock proteinHsp90

Identifiers

PMID41310205
PMCPMC12660321

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.