Evidence map›Paper›PMID 41309668›Full record

ArticleNPJ Regenerative medicine2025

Label-free in-line characterization of immune cell culture using quantitative phase imaging.

Caroline E Serafini, Viswanath Gorti, Paloma Casteleiro Costa, Aaron D Silva Trenkle, Bharat Kanwar, Bryan Wang, Brian Wicker, Linda E Kippner, Isaac LeCompte, Rui Qi Chen and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ Regenerative medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Qualitative and quantitative assessment ofBiomedical optics express · 2026
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Caroline E SerafiniGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Viswanath GortiThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Paloma Casteleiro CostaThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Aaron D Silva TrenkleThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Bharat KanwarGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Bryan WangThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Brian WickerMarcus Center for Therapeutic Cell Characterization and Manufacturing (MC3M), Georgia Institute of Technology, Atlanta, GA, USA.
Linda E KippnerMarcus Center for Therapeutic Cell Characterization and Manufacturing (MC3M), Georgia Institute of Technology, Atlanta, GA, USA.
Isaac LeCompteThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Rui Qi ChenH. Milton Stewart School of Industrial and Systems Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Benjamin JoffeGeorgia Tech Research Institute, Atlanta, GA, USA.
Ye LiMarcus Center for Therapeutic Cell Characterization and Manufacturing (MC3M), Georgia Institute of Technology, Atlanta, GA, USA.
Annie C Bowles-WelchMarcus Center for Therapeutic Cell Characterization and Manufacturing (MC3M), Georgia Institute of Technology, Atlanta, GA, USA.
Jing LiGeorgia Tech Research Institute, Atlanta, GA, USA.
Christine E BrownDepartment of Hematology & Hematopoietic Cell Transplantation (T Cell Therapeutics Research Laboratories), City of Hope Beckman Research Institute and Medical Center, Duarte, CA, USA.
Gabriel A KwongThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Stephen BalakirskyGeorgia Tech Research Institute, Atlanta, GA, USA.
Krishnendu RoyThe Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Francisco E RoblesGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA. robles@gatech.edu.

Funding

T32 CTEng (Cellular and Tissue Engineering) Training ProgramT32GM145735 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI Edward A. Botchwey, Andres J Garcia · 2022 to 2026
$2.3M
DNA-gated cytometry for multiplexed sorting of antigen-specific CD8 T cellsR01AI171892 · NIAID · GEORGIA INSTITUTE OF TECHNOLOGY · PI KWONG, GABRIEL A · 2022 to 2025
$2.0M
Accessible label-free optical microscopy with quantitative molecular and functional contrastR35GM147437 · NIGMS · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI Francisco E Robles · 2022 to 2026
$1.9M
Burroughs Wellcome Fund CASI BWF 1014540National Institute of Allergy and Infectious Diseases R01AI171892National Institutes of Health Cell and Tissue Engineering Training Program T32GM145735National Science Foundation Graduate Research Fellowship Program NSF GRFP DGE-2039655NIAID NIH HHS R01 AI171892NIGMS NIH HHS R35 GM147437NIGMS NIH HHS R35GM147437NIGMS NIH HHS T32 GM145735
6 · The paper itself

Abstract

Cell therapies, such as T cell immunotherapies, hold significant promise for treating complex diseases; however, their widespread adoption has been hindered by challenges related to monitoring cells during culture, which has affected their consistency, potency, and cost. Here, we present a compact, low-cost, label-free quantitative phase imaging (QPI) platform to enable continuous, non-destructive, in-line monitoring of T cell cultures within bioreactors. We further develop quantitative, image-based assays that accurately characterizes T cell culture viability and activation from over 50 independent donors-including therapeutically relevant CAR-T cells - while also preserving culture sterility and eliminating the need for disruptive sampling and endpoint assays. Our findings establish a QPI-pipeline for label-free, in-line cell monitoring and characterization which can significantly improve cell manufacturing processes.

Identifiers

PMID41309668
PMCPMC12660828

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.