Evidence map›Paper›PMID 41309554›Full record

ArticleNature communications2025

An antagonistic epigenetic mechanism regulating gene expression in pollen revealed through single-nucleus multiomics.

Colette L Picard, Lucia Ichino, Tyler J Buckley, Brandon A Boone, Jaewon Yun, Kevin D Abuhanna, Yi Zhang, Noah J Behrendt, Su May Lei Soe, Chongyuan Luo and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Colette L Picard *Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-2177-2216
Lucia Ichino *Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-0559-1626
Tyler J BuckleyDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-6015-9801
Brandon A BooneDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-6475-8000
Jaewon YunDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.
Kevin D AbuhannaDepartment of Human Genetics, University of California Los Angeles, Los Angeles, CA, USA.
Yi ZhangDepartment of Human Genetics, University of California Los Angeles, Los Angeles, CA, USA.
Noah J BehrendtDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.
Su May Lei SoeDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0009-0005-9934-099X
Chongyuan LuoDepartment of Human Genetics, University of California Los Angeles, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-8541-0695
Steven E JacobsenDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA. jacobsen@ucla.edu.ORCID http://orcid.org/0000-0001-9483-138X

Funding

Characterization of the function of gene body DNA methylationF32GM136115 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PICARD, COLETTE LAFONTAINE · 2020 to 2022
$201k
National Science Foundation (NSF) DGE-2034835, DGE-2444110NIGMS NIH HHS F32 GM136115U.S. Department of Health & Human Services | National Institutes of Health (NIH) F32GM136115
6 · The paper itself

Abstract

Arabidopsis MBD5, MBD6, and MBD7 are CG-specific methyl-readers with opposite functions: MBD5 and MBD6 (MBD5/6) repress methylated loci in pollen vegetative nuclei (VN), while MBD7 prevents transgene silencing, possibly by promoting DNA demethylation. Here we show that loss of MBD7 rescues transcriptional defects at a large subset of MBD5/6-bound loci. Using simultaneous profiling of DNA methylation and transcription in single pollen nuclei, we found that MBD5/6-bound loci that are actively demethylated in immature VN lose additional methylation in mbd5/6, prior to transcriptional derepression. A subset of these loci is also bound by MBD7, correlating with demethylation and transcriptional derepression in mbd5/6 that are both reversed by loss of MBD7. Conversely, ectopically recruiting the MBD7 complex to MBD5/6 targets causes partial demethylation and upregulation. We propose that MBD5/6 maintain silencing in VN in part by preventing the MBD7 complex from enhancing the active demethylation that occurs during VN maturation.

Indexed as

ArabidopsisArabidopsis ProteinsCell NucleusEpigenesis, GeneticGene Expression Regulation, PlantPollenDNA-Binding ProteinsDNA MethylationGene SilencingMultiomicsArabidopsis ProteinsDNA-Binding Proteins

Identifiers

PMID41309554
PMCPMC12660930

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.