Evidence map›Paper›PMID 41308953›Full record

ArticleVirus research2025

A novel monoclonal antibody targeting a conserved inner region of the hepatitis B virus envelope enables broad detection of immune escape variants.

Yansong Chen, Xinyu Zhang, Asha Ashuo, Zhong Fang, Wuhui Song, Jiangxia Liu, Jieliang Chen, Yaming Li, Zhenghong Yuan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yansong ChenKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Xinyu ZhangKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Asha AshuoKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Zhong FangLiver Cancer Institute of Zhongshan Hospital and Key Laboratory of Carcinogenesis and Cancer Invasion, Fudan University, Shanghai 200032, China.
Wuhui SongKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Jiangxia LiuKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Jieliang ChenKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. Electronic address: jieliangchen@fudan.edu.cn.
Yaming LiKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. Electronic address: yaming_li@fudan.edu.cn.
Zhenghong YuanKey Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. Electronic address: zhyuan@shmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The detection of hepatitis B surface antigen (HBsAg) is fundamental for the diagnosis of chronic hepatitis B (CHB). However, current diagnostic assays that rely on antibodies targeting the conformational "a" determinant of HBsAg are frequently compromised by mutations in this region, leading to undetected immune escape variants. In this study, we generated a novel monoclonal antibody, designated S1705, by immunizing mice with CHO-derived HBsAg. This antibody was identified to recognize a conserved linear epitope located within the internal loop region of the HBsAg particle. Western blot analysis under denaturing conditions confirmed that S1705 robustly detects HBsAg from multiple genotypes (A-D) and common clinical mutants, including the prevalent G145R variant. Moreover, S1705 demonstrated effective utility in applications such as flow cytometry and immunofluorescence. Notably, it exhibited superior performance compared to commercial antibodies targeting conformational epitopes in detecting HBsAg escape variants. We conclude that S1705, by targeting a conserved linear epitope, enables broad and reliable detection of both wild-type and mutant HBsAg. Critically, our findings demonstrate that immunodominant antigenic regions exist beyond the conventional boundaries of the 'a' determinant. This antibody thus holds significant promise for enhancing the diagnostic coverage of HBV variants and supporting future antiviral research and clinical monitoring.

Indexed as

Antibodies, MonoclonalHepatitis B AntibodiesHepatitis B Surface AntigensHepatitis B virusImmune EvasionAnimalsCHO CellsCricetulusEpitopesFemaleHepatitis BHepatitis B, ChronicHumansMiceMice, Inbred BALB CAntibodies, MonoclonalEpitopesHepatitis B AntibodiesHepatitis B Surface AntigensHBsAgHepatitis B virusImmune escapeInternal loopMonoclonal antibodyWestern blot

Identifiers

PMID41308953
PMCPMC12702348

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.