Evidence map›Paper›PMID 41307968›Full record

ArticleJournal of extracellular vesicles2025

Extracellular Vesicles Define Discrete Nano-Based Niches Within the Human Haematopoietic System.

Isabelle J Grenier-Pleau, Christopher J Wells, Samantha M Holmes, Christine Hall, Michael Vermeulen, Camille A de Villiers, Jelle Penders, Simon Vilms Pedersen, Sarah A Dick, Eric Bonneil and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Isabelle J Grenier-PleauDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Christopher J WellsDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Samantha M HolmesDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Christine HallDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Michael VermeulenDepartment of Biology, Queen's University, Kingston, Ontario, Canada.
Camille A de VilliersSPARTA Biodiscovery, London, UK.
Jelle PendersSPARTA Biodiscovery, London, UK.
Simon Vilms PedersenDepartment of Green Technology, University of Southern Denmark, Odense, Denmark.
Sarah A DickDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Eric BonneilInstitute for Research in Immunology and Cancer, Université de Montréal, Montréal, Quebec, Canada.
Mykhaylo SlobodyanyukComputational Biology Program, Ontario Institute for Cancer Research, 661 University Ave Suite 510 and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
Murtaza S NagreePrincess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada.
Jasleen KaurDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Amy J M McNaughtonDepartment of Pathology and Molecular Medicine, Queen's University, Kingston, Ontario, Canada.
Jamie BeaulieuInstitute for Research in Immunology and Cancer, Université de Montréal, Montréal, Quebec, Canada.
Molly M StevensDepartment of Materials, Department of Bioengineering, Institute for Biomedical Engineering, Imperial College London, London, UK.
Stephanie Z XiePrincess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada.
Michael J RauhDepartment of Pathology and Molecular Medicine, Queen's University, Kingston, Ontario, Canada.
Lynne-Marie PostovitDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
David J H F KnappInstitute for Research in Immunology and Cancer, Université de Montréal, Montréal, Quebec, Canada.
Jüri ReimandComputational Biology Program, Ontario Institute for Cancer Research, 661 University Ave Suite 510 and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
Kathrin TyryshkinDepartment of Pathology and Molecular Medicine, Queen's University, Kingston, Ontario, Canada.
Pierre ThibaultInstitute for Research in Immunology and Cancer, Université de Montréal, Montréal, Quebec, Canada.
Andrew W CraigDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
John F RudanDepartment of Surgery, Queen's University, Kingston, Ontario, Canada.
Steve MannDepartment of Surgery, Queen's University, Kingston, Ontario, Canada.
Edmond Y W ChanDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Sheela A AbrahamDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.ORCID https://orcid.org/0000-0003-4114-138X

Funding

CIHR 175484CIHR 394568Terry Fox New Investigator 1133The Terry Fox New Frontiers Program 6039065
6 · The paper itself

Abstract

Stem cell niches are complex multi-signalling networks comprised of molecular cues and physical interactions, orchestrated by niche-resident cells and the extracellular factors they produce. The bone niche specifically houses haematopoietic stem cells (HSCs), a critical cell type responsible for producing all blood and immune cells throughout life. Currently, how niches facilitate an ideal environment with simultaneously coordinating both intrinsic and extrinsic cellular signals is unknown. Studies presented here identify the existence of unique extracellular vesicle (EV)-defined niches within the haematopoietic system of human individuals. Bridging studies using proteomic signatures, nanoparticle characterization at single-vesicle resolution and machine learning-based techniques reveal that EVs can be grouped by blood, bone marrow and trabeculae within a human individual. Stem cell assays demonstrate that these niche-defined EVs impart functional effects on stem cells/progenitors based on location within the haematopoietic system. Finally, using single-cell transcriptomic analyses, results identify for the first time how niche-sourced EVs differentially affect the most primitive human HSCs and progenitors. This study highlights the significance of nanoparticles on human immunity and blood production and provides evidence for a new role for EVs, namely the demarcation of distinct nano-niches within biological systems.

Indexed as

Extracellular VesiclesHematopoietic Stem CellsStem Cell NicheHumansNanoparticlesProteomics

Identifiers

PMID41307968
PMCPMC12659769

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.