Evidence map›Paper›PMID 41307451›Full record

ArticleVirulence2025

Negative regulation of reassortant canine influenza virus replication and key site identification in porcine and ferret bronchial epithelial cell lines.

Jingjing Guo, Bud Jung, Sun-Woo Yoon, Yongjie Liu, Zhixin Feng, Minjoo Yeom, Woonsung Na, Qi Xu, Jong-Woo Lim, Maoda Pang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virulence, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jingjing GuoJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Bud JungDepartment of Pharmacy, Korea University, Sejong, Republic of Korea.
Sun-Woo YoonCollege of Life Sciences and Health Welfare, Department of Vaccine Biotechnology, Andong National University, Andong-si, Republic of Korea.ORCID 0000-0002-1448-4494
Yongjie LiuJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.ORCID 0000-0001-7301-9501
Zhixin FengJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Minjoo YeomCollege of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
Woonsung NaCollege of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Qi XuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China.
Jong-Woo LimCollege of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
Maoda PangKey Laboratory of Control Technology and Standard for Agro-Product Safety and Quality, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Fei HaoJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Rong ChenJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Daesub SongCollege of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
Xing XieJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.ORCID 0000-0003-0443-1046

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The segmented nature and high mutability of the influenza virus RNA genome facilitate rapid mutation and reassortment, allowing the virus to breach host barriers and migrate between different species, potentially leading to unpredictable influenza outbreaks. With dogs emerging as new natural hosts for influenza virus, vigilant surveillance and scientific prevention strategies are imperative. Here, based on our previous isolation of 21 strains, which are reassortments of the canine influenza virus (CIV) H3N2 (KR/07) with gene segments from the influenza A pandemic (H1N1) 2009 virus strain (CA/09), the replication kinetics of these reassortants in immortalized mammalian respiratory epithelial cell lines from swine and ferret named hTERT-PBECs and hTERT-FBECs, alongside induced changes in cytokine expression, were investigated. Reverse genetics was utilized to generate the reassortment H3N2 canine influenza rKR/07-PB2/NP, which contains the PB2 and NP segments from CA/09. The viral titer of rKR/07-PB2/NP was significantly lower than those of the parental viruses KR/07 and CA/09. In addition, rKR/07-PB2/NP notably decreased expression levels of interleukin-1β (IL-1β) and interleukin-10 (IL-10) in both immortal cells, particularly in hTERT-PBECs. Our findings not only contribute to the understanding and exploring cross-species transmission mechanisms of influenza virus, but also provide new ideas for prevention and treatment of CIV.

Indexed as

Epithelial CellsInfluenza A Virus, H3N2 SubtypeReassortant VirusesVirus ReplicationAnimalsBronchiCell LineCytokinesDogsFerretsInfluenza A Virus, H1N1 SubtypeOrthomyxoviridae InfectionsSwineCytokinesCanine influenza viruscytokine expressiongene segment reassortmentmammalian respiratory epithelial cell linesreverse geneticsviral replication

Identifiers

PMID41307451
PMCPMC12674360

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.