ArticleMolecular plant pathology2025
NtCML19 Is Recruited by Tobacco to Interact With the Deacetylase Protein RsDN3377 of Rhizoctonia solani AG3-TB, Inhibiting Fungal Infection.
Article in Molecular plant pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- NtCML19 Is Recruited by Tobacco to Interact With the Deacetylase Protein RsDN3377 of Rhizoctonia solani AG3-TB, Inhibiting Fungal Infection.Molecular plant pathology · 2025Article
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8 authors.
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Abstract
The necrotrophic fungal pathogen Rhizoctonia solani anastomosis group 3 (AG3-TB) is a major cause of global tobacco crop yield losses. Secreted proteins produced by filamentous fungi, as important virulence factors, play a core role in the interaction between plants and pathogens. In this study, we identified a secretory protein, RsDN3377, which localised to the intercellular space and induced cell death in Nicotiana benthamiana. Heterologous expression in Escherichia coli coupled with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry analysis confirmed RsDN3377 possessed deacetylase activity. In addition, RsDN3377 was an essential pathogenicity factor for mycelial development by double-stranded RNA-mediated gene silencing. Through yeast two-hybrid and bimolecular fluorescence complementation assays, we demonstrated that RsDN3377 interacted with the calcium-binding protein NtCML19. In addition, transgenic Yunyan 87 overexpressing NtCML19 exhibited enhanced resistance to R. solani AG3-TB infection. Microscale thermophoresis analysis verified the calcium-binding activity of NtCML19. These lines of evidence indicate that the deacetylase RsDN3377 is secreted by R. solani AG3-TB, and this protein, critical for promoting fungal mycelial development and pathogenicity, was disrupted by its resistance-related interaction with NtCML19.
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