ArticleBlood red cells & iron2025
Comprehensive Phenotypic and Proteomic Analyses of Human Reticulocyte Maturation.
Article in Blood red cells & iron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Tropism ofEmerging microbes & infections · 2026Article
- Multiomics analysis of red blood cells reveals thalassemia severity beyond globin gene mutations.Blood advances · 2026Article
- Tumor-derived DNA drives cancer-associated anemia by promoting reticulocyte clearance.Signal transduction and targeted therapy · 2026Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Reticulocyte maturation, the final stage of erythropoiesis following enucleation, is a dynamic 48- to 72-hour process remodeling multi-lobular, motile, and fragile nascent reticulocytes into deformable, biconcave red blood cells. This maturation involves organelle clearance and extensive membrane remodeling. A significant knowledge gap in our understanding is a lack of knowledge of the proteins involved in the earliest stages of reticulocyte maturation, the nascent bone marrow reticulocyte. To address this, we isolated and characterized the proteomic profiles of distinct reticulocyte populations from healthy human bone marrow. Density gradient separation, FACS analyses, and electron microscopy revealed a continuum of different stages of reticulocyte maturation in the marrow. Deformation-induced micropipette aspiration analyses combined with imaging of membrane proteins labeled with specific fluorescent probes, performed to better understand mechanisms of decreased expression of surface proteins during reticulocyte maturation, revealed a correlation between the extent of linkage and the decreased expression of surface proteins during reticulocyte maturation. There was a marked decrease in the total protein content between nascent and mature reticulocytes (56 to 39pg/cell) with a modest decrease between mature reticulocytes and mature erythrocytes (39 to 37pg/cell). Comparison of proteomes from nascent reticulocytes (2532 unique proteins), mature reticulocytes, and mature erythrocytes revealed only 867 proteins were shared among the three populations with significant changes in many classes of proteins. These studies provide comprehensive insights into maturation of the human reticulocyte proteome, advancing our understanding of the molecular mechanisms governing this process, and offer potential implications for acquired and inherited red blood cell disorders.
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Registered trials
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