Development of Immunodetection Systems Using a Specific Antibody Against the Recombinant Coat Protein for Detecting Sugarcane Streak Mosaic Virus.

Intan Ria Neliana, Bambang Sugiharto, Rikno Harmoko, Wahyu Indra Duwi Fanata

Abstract read

In one paragraph

Article in Pathogens (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Intan Ria NelianaPostgraduate Program in Biotechnology, University of Jember, Jl. Kalimantan No. 37, Kampus Tegalboto, Jember 68121, Indonesia.

Bambang SugihartoPostgraduate Program in Biotechnology, University of Jember, Jl. Kalimantan No. 37, Kampus Tegalboto, Jember 68121, Indonesia.ORCID 0000-0002-8125-1893

Rikno HarmokoCenter for Genetic Engineering, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, Cibinong, Bogor 16911, Indonesia.ORCID 0000-0003-3166-2966

Wahyu Indra Duwi FanataDoctoral Program in Agrultural Sciences, Faculty of Agriculture, University of Jember, Jl. Kalimantan No. 37, Kampus Tegalboto, Jember 68121, Indonesia.

Funding

Indonesian Ministry of Higher Education, Science, and Technology 3132/UN25.3.1/LT/2023RIIM batch 9th by the National Research and Innovation Agency 14970/UN25/KP/2022

6 · The paper itself

Abstract

Sugarcane streak mosaic virus (SCSMV) is one of the mosaic viruses found in mixed infection with two or more viruses. Infections of mosaic viruses show highly similar mosaic symptoms and are difficult to distinguish. This study aimed to develop a specific antibody against SCSMV that could potentially be utilized to differentiate mosaic virus infections. The cDNA encoding the coat protein (CP) of SCSMV was isolated by RT-PCR from the symptomatic sugarcane leaves. The cDNA was then used for the production of CP for the development of its polyclonal antibody. The nucleotide sequence of the cDNA showed high homology of 94.2-97.3% at the amino acid level with the

Indexed as

Antibodies, ViralCapsid ProteinsPlant DiseasesPotyviridaeSaccharumAnimalsPlant LeavesPotyvirusRabbitsRecombinant ProteinsAntibodies, ViralCapsid ProteinsRecombinant Proteinsantibodycoat proteinELISAimmunoblotimmunocapture RT-PCRSCSMVsugarcane

Identifiers

PMID41305344

DOI10.3390/pathogens14111106

PMCPMC12655272

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.