Assessment of Estrogenic and Genotoxic Activity in Wastewater Using Planar Bioassays.

Markus Windisch, Valentina Rieser, Clemens Kittinger

Abstract read

In one paragraph

Article in Toxics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Markus WindischDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz, 8010 Graz, Austria.

Valentina RieserDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz, 8010 Graz, Austria.

Clemens KittingerDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Diagnostic and Research Center for Molecular Biomedicine, Medical University of Graz, 8010 Graz, Austria.ORCID 0000-0001-8472-2155

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The contamination of ground and surface waters with micropollutants like estrogenic compounds and genotoxins is a major public health concern. Conventional wastewater treatment plants are currently not capable of completely removing those contaminants. In this study, we applied planar bioassays to investigate the genotoxicity and estrogenic activity of hospital and municipal wastewater from an Austrian treatment plant. Using the open-source 2LabsToGo platform in combination with the HPTLC-SOS-UmuC and HPTLC-YES assays, both genotoxic and estrogenic compound zones were detected in untreated wastewater. Genotoxic activity was found in sewage sludge filtrate and hospital wastewater, with bioanalytical concentrations ranging from 1.6 to 21.8 µg 4-NQO-EQ L

Indexed as

effect-directed analysisestrogenicitygenotoxicityhigh-performance thin-layer chromatographywastewater

Identifiers

PMID41304488

DOI10.3390/toxics13110936

PMCPMC12656073

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.