Evidence map›Paper›PMID 41304277›Full record

ArticleMicroorganisms2025

Crystal Digital PCR™ Enables Precise Quantification of Species Abundance in Microbial Mixtures.

Louis Delecourt, Mila Reset, Lionel Bertaux, James Sturgis, Yann Denis, Marie-Thérèse Giudici-Orticoni, Magali Roger, Christophe Bordi

Abstract read
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Louis DelecourtLISM, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.
Mila ResetLISM, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.
Lionel BertauxLCB, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.ORCID 0000-0002-2343-0252
James SturgisLISM, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.ORCID 0000-0001-5125-7699
Yann DenisPlatform Transcriptome, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.ORCID 0000-0003-0820-7447
Marie-Thérèse Giudici-OrticoniBIP, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.
Magali RogerBIP, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.ORCID 0000-0001-9696-2762
Christophe BordiLISM, IMM, Aix-Marseille Univ and CNRS, 13402 Marseille, France.ORCID 0000-0002-2877-6068

Funding

Agence Nationale de la Recherche ANR-22-PEBB-0002CNRS-Africa JPR 001
6 · The paper itself

Abstract

Accurate bacterial quantification is critical in many biological fields, from clinical diagnostics to environmental microbiology. Here, we establish a robust workflow for absolute quantification of bacterial species within mixed communities using Crystal Digital PCR

Indexed as

bacterial countingconsortiumdigital PCRquantification of mixed populations

Identifiers

PMID41304277
PMCPMC12654214

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.