ArticleMicroorganisms2025
Performance of Dried Blood Spot Cards for Serologic Detection of HPV16 Antibodies in Oropharyngeal Squamous Cell Carcinoma Patients.
Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Low HPV16 E6 Seroprevalence in HNSCC: A Prospective Study in Brazil.Journal of clinical medicine · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Antibodies against the human papillomavirus type 16 (HPV16) E6 oncoprotein are promising biomarkers for the early detection of HPV-driven oropharyngeal squamous cell carcinoma (HPV+OPSCC). Standard serologic testing is challenging in underserved regions with high HPV+OPSCC incidence. Dried blood spot (DBS) cards offer a low-resource alternative but remain unevaluated for HPV antibody detection. A total of 25 OPSCC patients who provided paired serum (venipuncture) and DBS (finger-prick) samples were recruited from the University of Kentucky. HPV16 antibodies (L1, E1, E2, E4, E6, E7) were measured using multiplex serology, quantified as median fluorescence intensity (MFI) and dichotomized using established cutoffs. Correlation between serum and DBS MFI values was evaluated using linear regression and Bland-Altman plots, whereas sensitivity, specificity, and Cohen's kappa assessed agreement. Mean MFI levels were lower in DBS than serum but were strongly correlated (R = 0.73 to 0.96; HPV16 E6 = 0.83). For HPV16 E6, DBS sensitivity was 90% (95% CI: 68-99) and specificity 100% (95% CI: 48-100), with kappa = 0.787. Specificity was 100% across all markers, while sensitivity varied from 0% (L1) to 100% (E2). DBS cards are accurate, inexpensive, and a scalable alternative for HPV16 E6 antibody detection, particularly in medically underserved regions, though further validation is needed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.