Foot Traffic Driven Anthropogenic Activity Alters Phyllosphere Microbial Community Characteristics and Putative Pathogens in Subtropical Urban Green Spaces.

Green spaces in subtropical cities are important for providing ecological services that support human well-being and serve as reservoirs for diverse microbial communities, which in turn support ecosystem functions. However, studies on the characteristics of the phyllosphere microbial community and public health risks associated with putative pathogens in various urban green spaces exposed to anthropogenic stress remain limited. To address this gap, we collected leaf samples from forests, greenbelts, parklands, and wetlands across Wuhan, China, and analyzed the bacterial and fungal communities via next-generation sequencing (NGS) techniques. For bacterial and fungal communities, alpha diversity was significantly greater in low-traffic zones than in high-traffic zones. Beta diversity analysis revealed distinct clustering of bacterial and fungal communities according to the urban green space type. Anthropogenic factors (foot traffic) influence green space type to shape microbial community structure, function, and stability, with shifts significantly associated with soil physicochemical properties via Mantel tests and redundancy analysis. The relative abundance of