Evidence map›Paper›PMID 41303702›Full record

ArticleInternational journal of molecular sciences2025

Validation of Housekeeping Genes for Normalizing RNA Expression in Real-Time PCR in Tuberculomas and Peripheral Blood Mononuclear Cells for Pulmonary Tuberculosis Patients.

Ekaterina K Tarasova, Ekaterina N Pavlova, Ekaterina Yu Rybalkina, Ekaterina A Scherbakova, Ruslan V Tarasov, Maria V Erokhina

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ekaterina K TarasovaCentral Tuberculosis Research Institute, 107564 Moscow, Russia.ORCID 0000-0003-0438-7233
Ekaterina N PavlovaFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.ORCID 0000-0001-5662-3715
Ekaterina Yu RybalkinaCentral Tuberculosis Research Institute, 107564 Moscow, Russia.
Ekaterina A ScherbakovaCentral Tuberculosis Research Institute, 107564 Moscow, Russia.
Ruslan V TarasovCentral Tuberculosis Research Institute, 107564 Moscow, Russia.ORCID 0000-0001-9498-1142
Maria V ErokhinaCentral Tuberculosis Research Institute, 107564 Moscow, Russia.ORCID 0000-0002-7256-4679

Funding

Russian Science Foundation 25-25-20207
6 · The paper itself

Abstract

Accurate normalization of qRT-PCR data in pulmonary tuberculosis (TB) research requires reference genes whose expression is invariant across clinically relevant matrices, yet no studies have addressed this in lesion tissue and blood concurrently. We assessed the expression stability of eight popular housekeeping genes-

Indexed as

Genes, EssentialLeukocytes, MononuclearReal-Time Polymerase Chain ReactionTuberculosis, PulmonaryAdultFemaleGene Expression ProfilingHumansMaleMiddle AgedReference Standardshousekeeping genesHPRT1PPIApulmonary tuberculosisvalidation of gene stabilityYWHAZ

Identifiers

PMID41303702
PMCPMC12653038

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.