Evidence map›Paper›PMID 41303352›Full record

ArticleInternational journal of molecular sciences2025

A Hydrogel Culture System Regulates Human Adipocyte Function.

Jason Junhyoung Kwon, Jie Li, Joshua Yu-Chung Liu, Christopher Patsalis, Peizi Wu, Yoshiki H Kawase, Alexander Ky, Carter Pasternak, Damian D Mason, Nadejda Bozadjieva-Kramer and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jason Junhyoung KwonDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Jie LiDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Joshua Yu-Chung LiuDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Christopher PatsalisDepartment of Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0009-0003-4585-0017
Peizi WuDepartment of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA.ORCID 0009-0003-9834-0176
Yoshiki H KawaseDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Alexander KyDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Carter PasternakDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Damian D MasonDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Nadejda Bozadjieva-KramerDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.ORCID 0000-0001-9796-2916
Claudia LoebelDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.ORCID 0000-0002-3140-5663
Carey N LumengDepartment of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Robert W O'RourkeDepartment of Surgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.ORCID 0000-0002-4038-4198

Funding

Regional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID P OLSON · 2013 to 2026
$24.3M
BLRD VA IK2 BX005715Grant from the David and Lucile Packard Foundation NA, (award to author to CL)NIDDK NIH HHS P30 DK020572NIH HHS 1R00HL151670-25NIH HHS 1R01DK090262-24NIH HHS 1R35GM157063-25NIH HHS 1R56DK132785-22VA Career Development Award from the United States Department of Veterans Affairs Biomedical Laboratory Research and Development Service IK2BX005715 (NBK)VA Merit Review Award from the United States Department of Veterans Affairs Clinical Sciences Research and Development Service I01CX001811 (RWO)
6 · The paper itself

Abstract

Alterations in the adipose tissue extracellular matrix are well established in obesity, but the role of matrix mechanics in regulating adipocyte function is not well understood. We used a hydrogel-adipocyte culture system to study the effects of matrix stiffness on adipocyte function. We found that intermediate matrix stiffness approximating native human adipose tissue promoted maximal adipogenesis and lipid storage, while stiffness above or below this threshold impaired adipogenesis and lipid storage. Transcriptomic analysis revealed that matrix stiffness regulated diverse signaling pathways in adipocytes related to lipid metabolism, immunity and inflammation, angiogenesis, and extracellular matrix interactions. These data elucidate the role of matrix mechanics in regulating adipocyte function and will guide further studies towards developing optimal matrix characteristics for adipocytes in in vitro culture models and designing adipocyte delivery vehicles for in vivo translational applications.

Indexed as

AdipocytesCell Culture TechniquesHydrogelsAdipogenesisAdipose TissueCells, CulturedExtracellular MatrixHumansLipid MetabolismSignal TransductionHydrogelsadipocyteadipogenesisextracellular matrixhydrogellipid storage

Identifiers

PMID41303352
PMCPMC12652782

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.