Evidence map›Paper›PMID 41302484›Full record

ArticleMolecules (Basel, Switzerland)2025

A Simplified Methodology for Solvent Screening in Selective Extraction of Lipids from Microalgae Based on Hansen Solubility Parameters.

Costas Tsioptsias, Stefania Mitis, Alexandra Rentzela, Kalitsa Alvanou, Dimitra Kelesi, Christos Manolis, Anastasia Stergiou, Sotirios D Kalamaras, Petros Samaras

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Costas TsioptsiasDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.ORCID 0000-0003-3847-0713
Stefania MitisDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.
Alexandra RentzelaDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.
Kalitsa AlvanouDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.
Dimitra KelesiDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.
Christos ManolisDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.ORCID 0009-0005-5008-8869
Anastasia StergiouDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.
Sotirios D KalamarasLaboratory of Animal Production and Environmental Protection, Faculty of Veterinary Medicine, School of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Petros SamarasDepartment of Food Science and Technology, Alexandrian University Campus at Sindos, International Hellenic University, 57400 Thessaloniki, Greece.ORCID 0000-0002-4081-8590

Funding

EU European Climate Infrastructure and Environment Executive Agency (CINEA) FUELPHORIA PN 101118286
6 · The paper itself

Abstract

Microalgae are considered a potential source of fatty acid esters that are suitable for biodiesel production. However, a principal bottleneck in lipids extraction is related to the selection of appropriate solvents in order to obtain an efficient process. In this work, a simple methodology based on Hansen Solubility Parameters (HSP) was developed, aiming to solvent screening towards selective extraction of lipid compounds: main parameters that were considered for an optimum solvent included the partitioning of free fatty acids and other non-desired solutes, e.g., pigments and phospholipids, as well as the minimum water dissolution. The method takes into account the affinity of a candidate solvent with desired and non-desired solutes along with their relative differences. A large number of solvents (>5000) were scanned by this method for their capacity to selectively extract fatty acid esters from microalgae biomass, and hexane proved to be among the optimum solvents. This prediction was supported by the Snyder's polarity index as well as ab initio quantum mechanical Density Functional Theory (DFT) calculations of the Gibbs free energy of solvation and partition coefficients. Moreover, model validation carried out by liquid-liquid extraction of algal liquor with hexane and other solvents, and measurement of lipids allocation using paper chromatography and spectroscopy. Low lipids yield was observed, while the extract was enriched in fatty acid esters. A critical discussion is provided regarding the low yield ratios and potential implications due to overestimation of lipids content in microalgae.

Indexed as

LipidsMicroalgaeSolventsBiomassEstersFatty AcidsLiquid-Liquid ExtractionSolubilityEstersFatty AcidsLipidsSolventsalgaebiodieselglyceridesHansen Solubility Parameterslipidsselective extractionsolvent extraction

Identifiers

PMID41302484
PMCPMC12655419

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.