Evidence map›Paper›PMID 41302204›Full record

ArticleLife (Basel, Switzerland)2025

Isolation and Characterization of Integrin α9 Positive Extracellular Vesicles Derived from Human Corneoscleral Rings.

Hung-Yin Lai, Ming-Chieh Hsieh, Hao-Hsiang Wu, Chien-Wei Lee, Shih-Hua Liu, Hsing-Yu Lin, Yi-Wen Chen, Chun-Chi Chiang, Yi-Ching Hsieh, Ying-Hsuen Wu and 4 more

Abstract read
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In one paragraph

Article in Life (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Hung-Yin LaiGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.ORCID 0000-0001-8405-4468
Ming-Chieh HsiehGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
Hao-Hsiang WuTranslational Cell Therapy Center, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.ORCID 0000-0002-0038-0459
Chien-Wei LeeTranslational Cell Therapy Center, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.ORCID 0000-0002-6679-0020
Shih-Hua LiuEye Center for Department of Ophthalmology, China Medical University Hospital, Taichung 404, Taiwan.
Hsing-Yu LinEye Center for Department of Ophthalmology, China Medical University Hospital, Taichung 404, Taiwan.
Yi-Wen ChenGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
Chun-Chi ChiangDepartment of Ophthalmology, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
Yi-Ching HsiehDepartment of Ophthalmology, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
Ying-Hsuen WuDepartment of Ophthalmology, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
You-Ling LiDepartment of Ophthalmology, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
Hsiao-Fan TungDepartment of Ophthalmology, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
Jennifer Hui-Chun HoDepartment of Ophthalmology, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
Yi-Yu TsaiDepartment of Ophthalmology, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.

Funding

China Medical University Hospital EXO-113-002
6 · The paper itself

Abstract

Corneoscleral-ring-derived extracellular vesicles represent a potential therapeutic strategy for promoting in vitro corneal wound healing. In this study, we successfully isolated and characterized extracellular vesicles from human corneolimbal tissue obtained from 42 donors, with a mean age of 51.62 ± 15.56 years. Donor-related factors such as age, corneal endothelial cell density, and underlying systemic conditions did not confound extracellular vesicle size and concentration with mean peak size of 99.52 ± 13.00 nm by nanoparticle tracking analysis. Western blotting analysis revealed positive Alix, stable expression of CD9 and CD81, and variable expression of CD63. Limbal stem cell (LSC)-associated markers, i.e., ABCG2, p63, Notch-1, and Integrin α9 were positively detected in the isolated extracellular vesicles. Notably, Integrin α9 showed stable and relatively strong expression in all samples serving a specific marker of LSC-derived extracellular vesicles. Functional assays demonstrated that LSC-derived extracellular vesicles exhibited better wound healing potency compared to extracellular vesicles derived from mesenchymal stem cells (MSCs). These findings suggest that corneoscleral-ring-derived extracellular vesicles express distinct LSC markers, including Integrin α9, and hold significant potential for application in corneal wound healing and ocular surface regeneration.

Indexed as

corneoscleral ringextracellular vesiclesIntegrin α9

Identifiers

PMID41302204
PMCPMC12654178

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.