ArticleBioengineering (Basel, Switzerland)2025
Spatial Regulation of Endocytosis and Adhesion Formation Governs Breast Cancer Cell Migration Under Confinement.
Article in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
8 authors.
Funding
Abstract
Cell migration through confined spaces is a critical step in cancer metastasis, yet the spatial regulation of endocytosis and adhesion dynamics during this process remains poorly understood. To investigate this, we adapted a microfluidic platform that generates stable, spatially linear biochemical gradients across 5 μm-tall migration channels. COMSOL simulations and optical calibration using FITC-dextran confirmed that gradients form reliably within 5 min. The microdevice also supports long-term live imaging and is compatible with both spinning disk confocal and total internal reflection fluorescence structured illumination microscopy modalities, enabling high-resolution visualization of adhesion and endocytic structures. By leveraging this platform for spatially restricted drug delivery, we locally applied the endocytic inhibitor Dyngo-4a to either the front or rear of migrating cells. This revealed that front-targeted endocytic inhibition preserved or increased leading-edge enrichment of paxillin and the clathrin adaptor AP-2, whereas rear-targeted inhibition eliminated paxillin polarity and reduced AP-2 polarity. These changes were accompanied by a significant increase in cell migration speed under front-targeted inhibition, while rear-targeted inhibition had no significant effect on speed and neither treatment altered persistence. Together, these findings suggest that endocytic polarity regulates adhesion dynamics and cell migration under confinement, offering a mechanistic insight into processes relevant to cancer cell invasion.
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Registered trials
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