Evidence map›Paper›PMID 41300801›Full record

ArticleGenes2025

Genome-Wide Identification of 13 miR5200 Loci in Wheat and Investigation of Their Regulatory Roles Under Stress.

Yuan Zhou, Chenyu Zhao, Huiyuan Yan, Jiahao Yang, Mingyang Chen, Xia Wang, Pingfan Xie, Yongjing Ni, Jishan Niu, Jiangping Ren and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yuan ZhouHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Chenyu ZhaoHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Huiyuan YanHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Jiahao YangHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Mingyang ChenHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Xia WangHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Pingfan XieHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Yongjing NiShangqiu Academy of Agricultural and Forestry Sciences, Shangqiu 476000, China.
Jishan NiuHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Jiangping RenHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Guojun XiaHenan Province Engineering Technology Research Center of High Resistance to Powdery Mildew Wheat, Zhengzhou 450046, China.
Yongchun LiHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Lei LiHenan Technology Innovation Centre of Wheat/National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450046, China.ORCID 0000-0002-1394-6335

Funding

Henan Province Science and Technology Research Project 252102111139the National Key R&D Program of China 2017YFD0301101the National Natural Science Foundation of China 32171972
6 · The paper itself

Abstract

BACKGROUND/

objectivesmiR5200 is miRNA unique to Poaceae plants. Induced under short-day conditions, it modulates flowering time by regulating the florigen

methodsThis study utilized wheat as the research material. First, through bioinformatics analysis at the genomic level, 13 potential candidate tae-miR5200 gene loci were screened. Subsequently, the authenticity of these gene loci was systematically validated by combining tobacco transient transfection-based GUS staining assay and quantitative real-time PCR (qRT-PCR) to detect expression levels. Building upon this foundation, the expression patterns of tae-miR5200 under abiotic stresses such as low temperature, drought, and salinity, as well as SA, ABA, IAA, GA

resultsExperimental validation confirmed that 7 out of 13 potential gene loci are authentic and functional, and tae-miR5200 exhibited specific expression changes under different types of abiotic stress.

conclusionsThis study confirms the authenticity of tae-miR5200 gene loci, effectively eliminating interference from bioinformatics-predicted false-positive loci in subsequent functional studies. It provides an experimental foundation for further investigation into the molecular mechanisms of tae-miR5200 in wheat responses to abiotic stress.

Indexed as

MicroRNAsStress, PhysiologicalTriticumGene Expression Regulation, PlantGenome, PlantMicroRNAsbioinformatics validationgene regulationmiR5200wheat stress response abiotic stress

Identifiers

PMID41300801
PMCPMC12652891

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.