ArticleBMC plant biology2025
A novel EST-SSR fingerprint database development strategy for accurate identification of asparagus cultivars.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Garden asparagus (Asparagus officinalis L.), a dioecious perennial crop, is highly valued for its nutritional and medicinal properties. The high similarity among cultivars and abundant intracultivar variation pose challenges to conventional cultivar identification methods. Leveraging the conserved parental genotype of 'clone hybrid' (CH) cultivars, this study employed a single-plant sampling strategy in which 20 individuals per cultivar were analysed to capture intracultivar variation. An innovative approach was further introduced for constructing a fingerprint database based on parental genotype data, which were reverse-inferred following Mendelian inheritance principles. Fifty EST-SSR markers, with an average polymorphism information content (PIC) of 0.6344, were selected to evaluate the genetic relationships among 62 cultivars. These cultivars were divided into three subgroups, revealing limited correlationt with their geographical origins and indicating their intricate genetic backgrounds. Furthermore, a core set of 7 EST-SSR markers effectively distinguished all cultivars except 'Huamiao' and 'C
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