Evidence map›Paper›PMID 41299253›Full record

ArticleBMC microbiology2025

High colonization by multidrug-resistant and virulent Staphylococcus aureus genotypes among critically ill patients in the COVID-19 pandemic in a Brazilian hospital.

Tamara Lopes Rocha De Oliveira, Thaís Campos Macharete, Evellyn Max Guedes, Gabriel Freire Igari, Andryelle Cristina de Sant'Ana, Adriana Lúcia Pires Ferreira, Fernanda Sampaio Cavalcante, Claudia Regina da Costa de Souza, Simone Aranha Nouér, Kátia Regina Netto Dos Santos and 1 more

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In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Tamara Lopes Rocha De Oliveira *Departamento de Microbiologia Médica, Laboratório de Infecção Hospitalar, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2‑010 ‑ Cidade Universitária, Rio de Janeiro, RJ, CEP 21941‑590, Brazil. tamara.lopes.oliveira@gmail.com.
Thaís Campos Macharete *Departamento de Microbiologia Médica, Laboratório de Infecção Hospitalar, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2‑010 ‑ Cidade Universitária, Rio de Janeiro, RJ, CEP 21941‑590, Brazil.
Evellyn Max GuedesDepartamento de Microbiologia Médica, Laboratório de Infecção Hospitalar, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2‑010 ‑ Cidade Universitária, Rio de Janeiro, RJ, CEP 21941‑590, Brazil.
Gabriel Freire IgariDepartamento de Microbiologia Médica, Laboratório de Infecção Hospitalar, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2‑010 ‑ Cidade Universitária, Rio de Janeiro, RJ, CEP 21941‑590, Brazil.
Andryelle Cristina de Sant'AnaDepartamento de Microbiologia Médica, Laboratório de Infecção Hospitalar, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2‑010 ‑ Cidade Universitária, Rio de Janeiro, RJ, CEP 21941‑590, Brazil.
Adriana Lúcia Pires FerreiraDepartamento de Doenças Infecciosas e Parasitárias, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Fernanda Sampaio CavalcanteDepartamento de Clínica Médica, Instituto de Ciências Médicas, Centro Multidisciplinar de Macaé, Universidade Federal do Rio de Janeiro, Macaé, Brazil.
Claudia Regina da Costa de SouzaDepartamento de Doenças Infecciosas e Parasitárias, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Simone Aranha NouérDepartamento de Doenças Infecciosas e Parasitárias, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Kátia Regina Netto Dos SantosDepartamento de Microbiologia Médica, Laboratório de Infecção Hospitalar, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Sala I2‑010 ‑ Cidade Universitária, Rio de Janeiro, RJ, CEP 21941‑590, Brazil. santoskrn@micro.ufrj.br.
Infection Control Group HUCFF/UFRJ

Funding

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro #E-26/203.296/2017; #E-26/200.419/2023, #E-26/010.000172/2016, #E-26/010.001463/2019, #E-26/010.101056/2018; #E-26/211.554/2019 (Projeto REDES); #E-26/201.071/2020; #E-26/211.284/2021; #E-26/201.454/2021 and #E-26/205.939/2022
6 · The paper itself

Abstract

backgroundThe COVID-19 pandemic has dramatically impacted in patients from Intensive Care Units (ICUs), making them more vulnerable to infection/colonization by clinically relevant pathogens. However, little is known about the Staphylococcus aureus isolates that colonized these patients during the pandemic.

methodsConsecutive S. aureus isolates from surveillance swabs of patients of two ICUs of a hospital in Rio de Janeiro, between September 2020 and September 2021, were evaluated for phenotypic antimicrobial resistance. Methicillin-resistant S. aureus (MRSA) isolates were evaluated for resistance and virulence genes, biofilm production, and their genotypic profiles. Medical records were accessed for clinical and demographic data.

resultsAmong 255 patients colonized by S. aureus, 79 (30.9%) were in the cICU (COVID-19 ICU) and 176 (69.1%) in the ncICU (non-COVID-19 ICU). COVID-19 patients made greater use of antimicrobials during hospitalization (p < 0.05). Higher rates of resistance for erythromycin and clindamycin, as well as the inducible macrolide-lincosamide-streptogramin B resistance phenotype and multidrug resistance were found in cICU isolates (p < 0.05). An overall rate of 36.5% of MRSA isolates was detected, with 41.7% in cICU and 34% in ncICU (p = 0.22). SCCmec types II (22.6%) and IV (71%) were the most found, and the latter was more frequent in the ncICU (p < 0.01). More than 50% of the isolates carried the resistance genes erm(C), msr(A), mrs(B), mph(C), aph(3')-III3a and smr, and the virulence genes icaA, sasG, ebpS, scn, egc cluster, fnbpB and cna. The cICU presented isolates that primarily carried the erm(C), smr, and cna genes and that showed strong biofilm production (p < 0.05). Clonal complex (CC) 5 was prevalent (65.6%) and associated with the hospital clones USA800/ST5-IV and USA100/ST105-II, which were most found in ncICU and cICU, respectively (p < 0.05). Community MRSA comprised 31.2% of isolates and mainly included the USA300/ST8-IV lineage (18.3%), with all isolates carrying the pvl genes.

conclusionsThe significant impact on antimicrobial resistance rates and the emergence of virulent lineages among S. aureus isolates during the COVID-19 pandemic highlights the close relationship between this disease and antimicrobial resistance and the importance of constant microbiological surveillance to reduce the risks associated with future pandemics.

Indexed as

COVID-19Drug Resistance, Multiple, BacterialStaphylococcal InfectionsStaphylococcus aureusAdultAgedAged, 80 and overAnti-Bacterial AgentsBiofilmsBrazilCritical IllnessFemaleGenotypeHumansIntensive Care UnitsMaleAnti-Bacterial AgentsVirulence FactorsAntimicrobial resistanceCOVID-19MRSA lineagesNasal colonizationS. aureusVirulence

Identifiers

PMID41299253
PMCPMC12751815

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.