Evidence map›Paper›PMID 41299226›Full record

ArticleBMC plant biology2025

Two WD40 proteins, AhWD40-170 and AhWD40-171, referred to regulation of anthocyanin accumulation in peanut (Arachis hypogaea L.).

Kun Zhang, Guanghao Wang, Hourui Shi, Chao Yin, Juntao Li, Juan Zhang, Yihe Cui, Jiaowen Pan, Jiayun Li, Gongshuai Wang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Drought Stress Mediated Changes in Food Crops: Mechanisms and Remediation Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Kun Zhang *College of Agricultural Science and Technology, Shandong Agriculture and Engineering University, Jinan, 250100, China.
Guanghao Wang *Institute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Hourui ShiShandong Seed Management Station, Jinan, 250100, China.
Chao YinTaian Institute for Food and Drug Control, Taian, 271000, China.
Juntao LiQU-Drake United College, Qingdao University, Qingdao, 266001, China.
Juan ZhangDongying Agricultural Comprehensive Service Center, Dongying, 257091, China.
Yihe CuiDongying Agricultural Comprehensive Service Center, Dongying, 257091, China.
Jiaowen PanInstitute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Jiayun LiInstitute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Jinan, 250100, China.
Gongshuai WangCollege of Agricultural Science and Technology, Shandong Agriculture and Engineering University, Jinan, 250100, China.
Jinghan YangCollege of Agricultural Science and Technology, Shandong Agriculture and Engineering University, Jinan, 250100, China. z2017036@sdaeu.edu.cn.
Chuanzhi ZhaoInstitute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Jinan, 250100, China. chuanzhiz@126.com.

Funding

Agricultural scientific and technological innovation project of Shandong Academy of Agricultural Sciences GXGC2025A07Funding project of New 20 Policies for Universities in Jinan 202333047Key Research and Development Project of Shandong Province 2025LZGC020, 2024TSGC0609, 2023TZXD078, 2024TSGC0789, 2024LZGC035Science and Technology Development Guidance Plan of Dongying Major Science and Technology Innovation Project 2024ZDJH100Shandong Agriculture and Engineering University Start-Up Fund for Talented Scholars 2024GCCZR-25Shandong Agriculture and Engineering University Young Teacher Scientific Research Project QNKJZ202308
6 · The paper itself

Abstract

backgroundWD40 proteins represent a major conserved eukaryotic family, participating broadly in regulating diverse biological processes and secondary metabolite biosynthesis, including anthocyanin production. While peanut (Arachis hypogaea) ranks among the world’s most significant oilseed crops, the AhWD40 family remains uncharacterized, with unknown regulatory roles in anthocyanin accumulation.

resultsOur genome-wide analysis identified 367 AhWD40 members. Bioinformatics characterization revealed both conserved features and subgroup-specific attributes among family members. Evolutionary analysis further indicated segmental duplications as principal drivers of AhWD40 family expansion. Ka/Ks calculations demonstrated dominant purifying selection during AhWD40 evolution. Notably, six members exhibit high sequence similarity to Arabidopsis TRANSPARENT TESTA GLABRA1 (TTG1). Transcriptomic and qRT-PCR analyses established strong correlations between AhWD40-170 and AhWD40-171 expression and anthocyanin accumulation. Transient overexpression of either gene in apple skin significantly enhanced anthocyanin biosynthesis.

conclusionThis study provides a comprehensive understanding of the structure, classification, evolution, and anthocyanin accumulation of WD40 genes in peanut. These findings establish a functional framework for AhWD40s in anthocyanin regulation and propose novel genetic targets for enhancing plant anthocyanin production.

Indexed as

AnthocyaninsArachisPlant ProteinsWD40 RepeatsGene Expression Regulation, PlantPhylogenyAnthocyaninsPlant ProteinsAnthocyanin biosynthesisArachis hypogaeaWD40 proteins

Identifiers

PMID41299226
PMCPMC12659237

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