ArticleBMC genomics2025
Tobacco rattle virus infection alters Arabidopsis gene expression via virus-derived SiRNAs and host MiRNA network disruption.
Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundVirus-induced gene silencing (VIGS) technology based on tobacco rattle virus (TRV) has been widely employed in plant gene function research. However, the regulatory roles of TRV-derived small interfering RNAs (siRNAs) in Arabidopsis gene expression and the impact of TRV infection on the endogenous microRNA (miRNA) regulatory network in Arabidopsis remain undefined.
resultsIn this study, many TRV-derived sRNAs were identified in infected Arabidopsis leaves. Target validation confirmed that 26 viral siRNAs specifically targeted 17 host mRNAs involved in critical biological processes, including chlorophyll biosynthesis, protein translation, metabolic homeostasis, and stress response pathways. Additionally, TRV infection induced the differential expression of 120 endogenous miRNAs (with 108 showing downregulation), disrupting flowering regulatory modules such as the miR156-SQUAMOSA promoter binding protein-like gene (SPL) and the miR172-APETALA2 (AP2), as well as miRNA regulatory networks associated with plant innate immunity and drought stress responses. Further investigations revealed that TRV infection significantly suppressed the expression of trans-acting small interfering RNAs(tasiRNAs)triggered by miR173, thereby affecting the regulation of genes involved in carbon metabolism and ethylene biosynthesis.
conclusionsThis study elucidates the broad-spectrum interference of TRV-siRNAs with host gene expression and the remodeling of plant endogenous miRNA/tasiRNA regulatory networks by TRV infection. It provides critical insights into the unintended impacts of TRV infection on plant systems and suggests that the inherent effects of TRV on gene expression and physiology should be carefully considered when employing TRV as a genetic engineering tool.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.