Assessment of cytotoxicity of some synthetic compounds against breast carcinoma spheroids with subsequent analysis of pro-apoptotic and gene expression.

Khaled Mahmoud, Elham M Youssef, Farid M Sroor, Walid Fayad

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Khaled MahmoudDrug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, Pharmaceutical and Drug Industries Division, National Research Centre, Dokki, Giza, 12622, Egypt. khaledmmh2003@yahoo.com.

Elham M YoussefBiochemistry Department, National Research Centre, Giza, Egypt.

Farid M SroorOrganometallic and Organometalloid Chemistry Department, National Research Centre, Cairo, 12622, Egypt.

Walid FayadDrug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, Pharmaceutical and Drug Industries Division, National Research Centre, Dokki, Giza, 12622, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The current study examined the anticancer potential of 80 synthetic organic compounds at 50µM using in vitro two-dimensional (2D) and three-dimensional (3D) spheroids model breast adenocarcinoma (MCF7) models. The most promising six cytotoxic compounds on the 3D spheroids model (> 70% growth cytotoxicity) were counter-screened for their safety on normal cells (RPE1 cell line) grown as 3D spheroids. In parallel, the six compounds were tested for their pro-apoptotic activity using the M30 Cyto death ELISA assay. Based on the activity on MCF7 3D spheroids model, half maximum inhibitory concentration (IC

Indexed as

Antineoplastic AgentsApoptosisBreast NeoplasmsGene Expression Regulation, NeoplasticSpheroids, CellularCell SurvivalDrug Screening Assays, AntitumorFemaleGene Expression ProfilingHumansMCF-7 CellsAntineoplastic Agents2D3D spheroids modelApoptotic genesBreast cancerInflammatorySynthetic compounds

Identifiers

PMID41298834

DOI10.1038/s41598-025-26942-w

PMCPMC12663132

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.