Evidence map›Paper›PMID 41298827›Full record

ArticleScientific reports2025

High connectivity and low differentiation of Plasmodium falciparum parasite populations in a setting with high seasonal migration.

Endashaw Esayas, William Louie, Isobel Routledge, Maxwell Murphy, Nigatu Negash Demeke, Faith De Amaral, Andrés Aranda-Díaz, Bryan Greenhouse, Fikregabrail Aberra Kassa, Tedros Nigusse and 8 more

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Genetic Diversity and Allelic Distribution ofJournal of parasitology research · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Endashaw Esayas *Malaria and Neglected Tropical Diseases Research Division, Armauer Hansen Research Institute, Addis Ababa, Ethiopia. esu.endo@gmail.com.
William Louie *EPPIcenter Research Program, Division of HIV, Infectious Diseases and Global Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.
Isobel Routledge *Malaria Elimination Initiative, Global Health Group, University of California San Francisco, San Francisco, CA, USA.
Maxwell MurphyEPPIcenter Research Program, Division of HIV, Infectious Diseases and Global Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.
Nigatu Negash DemekeMalaria and Neglected Tropical Diseases Research Division, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
Faith De AmaralEPPIcenter Research Program, Division of HIV, Infectious Diseases and Global Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.
Andrés Aranda-DíazEPPIcenter Research Program, Division of HIV, Infectious Diseases and Global Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.
Bryan GreenhouseEPPIcenter Research Program, Division of HIV, Infectious Diseases and Global Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.
Fikregabrail Aberra KassaMalaria and Neglected Tropical Diseases Research Division, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
Tedros NigusseMalaria and Neglected Tropical Diseases Research Division, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
Muluken AssefaMalaria and Neglected Tropical Diseases Research Division, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
Temesgen AshineMalaria and Neglected Tropical Diseases Research Division, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
Asefaw GetachewPATH Malaria Control and Elimination Partnership in Africa (MACEPA), Seattle, USA.
Henry NtukuPATH Malaria Control and Elimination Partnership in Africa (MACEPA), Seattle, USA.
Lemu GolassaAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Endalamaw GadisaMalaria and Neglected Tropical Diseases Research Division, Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
Adam BennettPATH Malaria Control and Elimination Partnership in Africa (MACEPA), Seattle, USA.
Jennifer L SmithEPPIcenter Research Program, Division of HIV, Infectious Diseases and Global Medicine, Department of Medicine, University of California, San Francisco, San Francisco, CA, USA.

Funding

Genetic and social network analysis to target interventions for malaria eliminationK01AI153555 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SMITH, JENNIFER LINNEA · 2020 to 2024
$725k
Gates Foundation INV-003212National Institute of Allergy and Infectious Diseases 5K01AI153555NIAID NIH HHS K01 AI153555
6 · The paper itself

Abstract

Seasonal movement of less-immune people from low- to high- transmission regions increase malaria risk and may introduce parasite strains to both areas. This study examined Plasmodium falciparum genetic diversity and connectivity between low-transmission highlands and endemic lowlands in Ethiopia to assess the contribution of seasonal agricultural migration in sustaining transmission. P. falciparum qPCR-positive dried blood spots collected from highland health facilities and lowland agricultural worksites were sequenced using multiplexed amplicon sequencing. Complexity of infection (COI) and infection pairwise relatedness were estimated and used for clustering analysis. Lowland populations (seasonal workers and residents) had higher COI and polyclonal infection rates (mean COI 2.62, 62%, n = 581) than highland residents (mean COI 2.00, 40%, n = 599). Similar expected heterozygosity (He ≈ 0.4) was observed, and P. falciparum infections from worksites showed high genetic connectivity between highland and lowland populations, with extensive parasite sharing, including 27 related clusters in highland cases and 10 in seasonal workers. Integrating parasite genomic data with epidemiological information revealed strong connectivity and low genetic differentiation between these regions linked by seasonal migration. These findings highlight how agricultural mobility likely drives parasite diversity and gene flow, implicating its role in sustaining malaria transmission.

Indexed as

Malaria, FalciparumPlasmodium falciparumAdultEthiopiaFemaleGenetic VariationHumansMaleSeasonsAmplicon sequencingComplexity of infectionEthiopiaGenetic connectivityHighland-lowland populationsMalariaPlasmodium falciparumSeasonal agricultural workers

Identifiers

PMID41298827
PMCPMC12749964

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.