Evidence map›Paper›PMID 41298762›Full record

ArticleScientific reports2025

Single-cell and bulk transcriptomics reveal Fusobacterium nucleatum promotes colorectal cancer metastasis by upregulating LAMC2 in in vitro and in vivo models.

Songlin Ran, Jinhua Ye, Hufei Wang, Fangzhou Liu, Suwen Ou, Zhiyong Chen, Yanni Song, Shu-Lin Liu, Rui Huang

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Songlin Ran *Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Jinhua Ye *Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Hufei Wang *Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Fangzhou Liu *Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Suwen OuDepartment of Pancreatic and Biliary Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
Zhiyong ChenDepartment of Gastroenterology, suizhou Hospital, Hubei University of Medicine, Sui Zhou, 441399, China.
Yanni SongDepartment of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 150081, China. yannimd@126.com.
Shu-Lin LiuGenomics Research Center, College of Pharmacy, Harbin Medical University, Harbin, 150081, China. slliu@hrbmu.edu.cn.
Rui HuangDepartment of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China. huangrui2019@163.com.

Funding

National Natural Science Foundation of China No. 81872034
6 · The paper itself

Abstract

Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide. Fusobacterium nucleatum (F. nucleatum) has been strongly implicated in CRC metastasis. However, the contribution of laminin γ2 chain (LAMC2) to F. nucleatum-induced CRC metastasis remains elusive. We investigated the role of LAMC2 in F. nucleatum-induced CRC cell migration through a series of in vitro assays, including transmission electron microscopy, wound-healing, and transwell migration experiments, as well as in vivo animal models. Additionally, we explored the expression profile of LAMC2 in CRC tissues and assessed its prognostic relevance using immunohistochemistry, bulk transcriptome, and single-cell transcriptomics analyses. We found that F. nucleatum upregulates LAMC2 expression, thereby promoting CRC cell migration. Mechanistic analyses revealed that F. nucleatum downregulates hsa-miRNA-7977, releasing its transcriptional repression of LAMC2. Additionally, LAMC2 activates the FAK-PI3K-AKT signaling pathway and modulates H3K27 histone acetylation. Immunohistochemistry, together with single-cell and bulk transcriptome analyses, demonstrated that LAMC2 is highly expressed in metastatic CRC tissues, and is significantly associated with poor prognosis. Moreover, a Cox regression-based prognostic model constructed using LAMC2-associated genes exhibited robust predictive performance across multiple independent cohorts. Our findings identify LAMC2 as a key mediator of F. nucleatum-induced CRC metastasis and highlight it as both a prognostic biomarker and a potential therapeutic target.

Indexed as

Colorectal NeoplasmsFusobacterium InfectionsFusobacterium nucleatumLamininTranscriptomeAnimalsCell Line, TumorCell MovementFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticHumansMaleMiceMicroRNAsNeoplasm MetastasisLAMC2 protein, humanLamininMicroRNAsColorectal cancerFusobacterium nucleatumHistone acetylationLAMC2Metastasis

Identifiers

PMID41298762
PMCPMC12657517

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