Evidence map›Paper›PMID 41298395›Full record

ArticleNature communications2025

Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity.

Akshita B Gopal, Huibin Lv, Tossapol Pholcharee, Wenhao O Ouyang, Qi Wen Teo, Yasha Luo, Yun Sang Tang, Mingyong Luo, Chris K P Mok, Nicholas C Wu

Abstract read
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In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Akshita B Gopal *Department of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Huibin Lv *Department of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA. huibinlv@illinois.edu.ORCID http://orcid.org/0000-0003-2218-0076
Tossapol PholchareeDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Wenhao O OuyangDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Qi Wen TeoDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.ORCID http://orcid.org/0000-0001-6749-9684
Yasha LuoDepartment of Clinical Laboratory, Guangdong Women and Children Hospital, Guangzhou, China.
Yun Sang TangThe Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, China.
Mingyong LuoDepartment of Clinical Laboratory, Guangdong Women and Children Hospital, Guangzhou, China.
Chris K P MokThe Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, China.ORCID http://orcid.org/0000-0002-0525-6772
Nicholas C WuDepartment of Biochemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA. nicwu@illinois.edu.ORCID http://orcid.org/0000-0002-9078-6697

Funding

Food and Health Bureau of the Government of the Hong Kong Special Administrative Region | Health and Medical Research Fund (HMRF) 24230352Howard Hughes Medical Institute (HHMI) Emerging Pathogens InitiativeKinship Foundation Searle Scholars ProgramResearch Grants Council, University Grants Committee (RGC, UGC) HKU C7053-24GUofI | University of Illinois at Urbana-Champaign (UIUC) Carl R. Woese Institute for Genomic Biology Postdoctoral FellowshipUofI | University of Illinois at Urbana-Champaign (UIUC) William T. and Lynn Jackson Graduate Student FellowshipVallee Foundation (Bert L. & N. Kuggie Vallee Foundation) Vallee Scholars Program
6 · The paper itself

Abstract

The conserved stem domain of influenza hemagglutinin (HA), which is classified into group 1 and group 2, is a target of broadly neutralizing antibodies. While many group 1 HA stem antibodies have been described, much less is known about group 2 HA stem antibodies. This study structurally characterizes two group 2 HA stem antibodies, 2F02 and AG2-G02, targeting the central stem epitope and the lower stem epitope, respectively. Unlike prototypic antibodies to group 2 HA stem, 2F02 and AG2-G02 do not compete for binding. Both antibodies offer protection in a female mouse model via neutralization activity and Fc-mediated effector functions. We further demonstrate that the natural evolution of HA2 position 32 restricts the binding of AG2-G02 to recent human H3N2 HAs and influences the binding of human plasma samples. Overall, these findings advance our understanding of the antigenicity of HA stem, which has important implications for the development of broadly protective influenza vaccines.

Indexed as

Antibodies, ViralAntigens, ViralHemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H3N2 SubtypeAnimalsAntibodies, NeutralizingEpitopesFemaleHumansInfluenza, HumanInfluenza VaccinesMiceMice, Inbred BALB COrthomyxoviridae InfectionsAntibodies, NeutralizingAntibodies, ViralAntigens, ViralEpitopesHemagglutinin Glycoproteins, Influenza VirusInfluenza Vaccines

Identifiers

PMID41298395
PMCPMC12658140

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.