Evidence map›Paper›PMID 41298232›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Single-Cell Metabolic Imaging Reveals Glycogen-Driven Adaptations in Endothelial Cells.

Rahuljeet S Chadha, Benjamin Yang, Dongqiang Yuan, Edmund D Kapelczak, Philip A Kocheril, Alonso Tapia, Shivansh Mahajan, Adrian Colazo, Naseeb K Malhi, Joseph A Ambarian and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Single-Cell Metabolic Imaging Reveals Glycogen-Driven Adaptations in Endothelial Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Rahuljeet S ChadhaDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.ORCID https://orcid.org/0000-0002-3805-6144
Benjamin YangDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Dongqiang YuanDepartment of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope Medical Center, Duarte, CA, 91010, USA.
Edmund D KapelczakDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA.ORCID https://orcid.org/0000-0002-6305-0983
Philip A KocherilDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Alonso TapiaDepartment of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope Medical Center, Duarte, CA, 91010, USA.ORCID https://orcid.org/0000-0002-3123-9159
Shivansh MahajanDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Adrian ColazoDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Naseeb K MalhiDepartment of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope Medical Center, Duarte, CA, 91010, USA.
Joseph A AmbarianDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Xuejing LiuDepartment of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope Medical Center, Duarte, CA, 91010, USA.
Tara A TeSlaaDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA.ORCID https://orcid.org/0000-0002-0504-0998
Zhen B ChenDepartment of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope Medical Center, Duarte, CA, 91010, USA.
Lu WeiDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.ORCID https://orcid.org/0000-0001-9170-2283

Funding

RNA-mediated endotheliopathy in diabetic vasculopathyR35HL171550 · NHLBI · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI Zhen Bouman Chen · 2024 to 2026
$2.2M
Chromatin remodeling factors and mechanisms in diabetic microvascular complicationsR01DK143577 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI Zhen Bouman Chen, RAMA NATARAJAN · 2025 to 2026
$1.5M
ACS Division of Analytical ChemistryAmerican Heart Association 24POST1195441American Heart Association 25POST1365287Chan Zuckerberg InitiativeHeritage Medical Research InstituteHertz FoundationNational Science Foundation DGE-1745301National Science Foundation DGE-2139433NHLBI NIH HHS R35 HL171550NIDDK NIH HHS R01 DK143577NIH HHS R35 HL171550
6 · The paper itself

Abstract

Endothelial dysfunction (ED) is a defining feature of diabetes mellitus (DM) and a key contributor to many metabolic and cardiovascular diseases. Endothelial cells (ECs) are known to be highly glycolytic and primarily rely on glucose to meet their energy demands. However, the role of glycogen metabolism in ECs remains poorly characterized due to a lack of suitable tools. Here, stimulated Raman scattering (SRS) microscopy is utilized to investigate glycogen metabolism in live ECs exposed to a diabetes-mimicking milieu-high glucose and tumor necrosis factor (TNF-α). It is shown that ECs divert excess glucose toward subcellular glycogen storage, and that this storage capacity is significantly enhanced by the inhibition of glycogen synthase kinase 3 (GSK3). Pulse-chase experiments uncover glycogen dynamics and reveal rapid mobilization under glucose starvation, highlighting its role as an immediate energy reserve. The capabilities of SRS imaging are further extended to visualize glutamine and lactate metabolism for the first time, directly showcasing EC reliance on alternative substrates during glucose deprivation. ECs containing glycogen exhibit reduced immediate metabolic demand for these gluconeogenic substrates. These findings suggest that glycogen may play a regulatory role in modulating stress-responsive metabolic adaptations and may offer therapeutic opportunities to address diabetes-induced ED and related cardiometabolic diseases.

Indexed as

Adaptation, PhysiologicalEndothelial CellsGlycogenSingle-Cell AnalysisGlucoseGlycolysisHumansSpectrum Analysis, RamanGlucoseGlycogenendothelial dysfunctionglutamine and lactate metabolismmetabolic imagingstimulated Raman imagingsubcellular glycogen

Identifiers

PMID41298232
PMCPMC12866687

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.