Evidence map›Paper›PMID 41297822›Full record

ArticleInternational journal of biological macromolecules2026

Ice-binding protein's dual roles for prolonging the enzymatic activity of alcohol dehydrogenase against freezing.

Daisy Aguirre, Isabella Nieblas, Alfredo Gonzalez, Emily L Zempel, Brenda A Rounds, Ting-Yu Wang, Tsui-Fen Chou, Ashley Cheng, Jaelyn Asamoah, Kenneth Liu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of biological macromolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Daisy AguirreDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Isabella NieblasDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Alfredo GonzalezDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Emily L ZempelDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Brenda A RoundsDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Ting-Yu WangProteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, CA, 91106, USA.
Tsui-Fen ChouProteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, CA, 91106, USA.
Ashley ChengDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Jaelyn AsamoahDepartment of Biological Sciences, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Kenneth LiuDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Aidan ReyesDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA.
Caitlin E ScottDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA. Electronic address: cscott37@calstatela.edu.
Xin WenDepartment of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA, 90032, USA. Electronic address: xwen3@calstatela.edu.

Funding

UNDERGRADUATE STUDENT TRAINING IN ACADEMIC RESEARCHT34GM008228 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI TUNSTAD, LINDA M. · 1986 to 2022
$13.3M
Bridges to the Doctorate Program at Cal State LAT32GM146700 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI Melanie J Cocco, Michael Lloyd Hayes · 2022 to 2026
$5.1M
Undergraduate Research Training for Student Enhancement (U-RISE) at Cal State LAT34GM145503 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI KRISHNA L FOSTER · 2022 to 2026
$3.7M
IDENTIFICATION AND MOLECULAR BASIS FOR EFFICIENT ANTIFREEZE PROTIEN ENHANCERSSC3GM086249 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI WEN, XIN · 2009 to 2023
$1.7M
NIGMS NIH HHS SC3 GM086249NIGMS NIH HHS T32 GM146700NIGMS NIH HHS T34 GM008228NIGMS NIH HHS T34 GM145503
6 · The paper itself

Abstract

As biological catalysts, enzymes are essential not only in life, but also various industrial processes. Preservation of enzymatic activity against freeze-thaw damage is critical for many industrial applications. As a type of ice-binding proteins, antifreeze proteins (AFPs) are notable for their ability to control ice growth and ice recrystallization inhibition (IRI). Additionally, AFPs were recently discovered to interact with sugars, nucleosides, and other proteins, making them remarkably attractive for biopreservation. Here, we characterize the role of DAFP-1, a recombinant insect AFP in preserving the activity of industrially important alcohol dehydrogenase (ADH) in dilute solutions at temperatures below freezing. The presence of DAFP-1 at a molar ratio of 4:1 effectively preserves ADH enzyme activity following multiple freeze-thaw cycles. The concentration of DAFP-1 for its IRI activity is substantially lower than that for optimal ADH activity preservation. Enzymatic assays and fluorescence spectroscopy suggest DAFP-1's effects on ADH's conformation around the Trp-His pairs close to its catalytic regions. The interaction between DAFP-1 and ADH is further elucidated by molecular docking and corroborated by molecular dynamics simulations. These findings reveal an AFP's dual protective roles in effective enzyme cryoprotection, highlighting the importance of molecular interactions in cryopreservation by AFPs for bioinspired design.

Indexed as

Alcohol DehydrogenaseAntifreeze ProteinsFreezingAnimalsIceMolecular Docking SimulationMolecular Dynamics SimulationProtein BindingRecombinant ProteinsAlcohol DehydrogenaseAntifreeze ProteinsIceRecombinant ProteinsEnzymatic activity preservationIce-binding (antifreeze) proteinProtein-protein docking and molecular dynamics simulations

Identifiers

PMID41297822
PMCPMC12747722

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.