Evidence map›Paper›PMID 41296729›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

The intermediate filament protein GFAP regulates mitochondrial fission in astrocytes.

Ding Xiong, Ye Sing Tan, Fang Yuan, Yinglu Li, Kay En Low, Isabelle Bonne, Sakthikumar Mathivanan, Phil Jun Kang, Zijun Sun, Xueyan Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The intermediate filament protein GFAP regulates mitochondrial fission in astrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Ding XiongProgram in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore 169857, Singapore.ORCID 0000-0002-2232-9013
Ye Sing TanProgram in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore 169857, Singapore.ORCID 0000-0002-4840-1853
Fang YuanProgram in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore 169857, Singapore.
Yinglu LiState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Kay En LowElectron Microscopy Unit, Microscopy Cluster, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117549, Singapore.
Isabelle BonneElectron Microscopy Unit, Microscopy Cluster, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117549, Singapore.ORCID 0000-0002-8261-6794
Sakthikumar MathivananCenter for Neurologic Diseases, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037.
Phil Jun KangProgram in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore 169857, Singapore.
Zijun SunWaisman Center, University of Wisconsin-Madison, Madison, WI 53705.
Xueyan LiWaisman Center, University of Wisconsin-Madison, Madison, WI 53705.
Emily AbellaWaisman Center, University of Wisconsin-Madison, Madison, WI 53705.
Albee MessingWaisman Center, University of Wisconsin-Madison, Madison, WI 53705.ORCID 0000-0001-6049-0521
Linghai KongWaisman Center, University of Wisconsin-Madison, Madison, WI 53705.ORCID 0000-0002-8015-5971
Su-Chun ZhangProgram in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore 169857, Singapore.ORCID 0000-0003-0563-5147

Funding

Patient-derived iPS cells as model systems for AxDP01HD076892 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI MESSING, ALBEE · 2014 to 2018
$6.0M
Aligning Science Across Parkinson's (ASAP) ASAP-000301Duke-NUS Medical School (DukeNUS) Duke-NUS-KPFA/2018/0022MOST | National Natural Science Foundation of China (NSFC) 82301042National Institute of Health HD076892National Institute of Health HD090256National Institute of Health HD106197NICHD NIH HHS P01 HD076892Sichuan Science and Technology Program 2023ZYD0065Singapore Ministry of Education MOE2018-T2-2-103Singapore Ministry of Health MOH-000207Singapore Ministry of Health MOH-000212
6 · The paper itself

Abstract

Mitochondrial plasticity, coordinated by fission and fusion, is crucial to ensure cellular functions. Mitochondrial fission is mediated by the GTPase Drp1 at the constriction site, which is proposed to be driven by the actin-myosin contractile force. However, the mechanism that propels constriction remains unclear, and the potential involvement of additional mechanisms in this process remains an open question. Here, using structured illumination microscopy, electron microscopy, and correlative light electron microscopy (CLEM), we show that the type III intermediate filament glial fibrillary acidic protein (GFAP) participates in mitochondria constriction and fission by interacting with Drp1. Remarkably, loss of GFAP results in hyperfused mitochondria under physiological and even Ca

Indexed as

AstrocytesGlial Fibrillary Acidic ProteinMitochondriaMitochondrial DynamicsAlexander DiseaseAnimalsCalciumDynaminsHumansIntermediate FilamentsMiceMitochondrial ProteinsCalciumDnm1l protein, mouseDynaminsGlial Fibrillary Acidic ProteinMitochondrial Proteinsalexander diseaseastrocyteGFAPintermediate filamentmitochondrial fission

Identifiers

PMID41296729
PMCPMC12685142

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.