Evidence map›Paper›PMID 41296720›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Probing direct interactions between nuclear proteins in cells with nxReLo.

Sherif Ismail, Jana Kubíková, Maria Maichel, Peter Andersen, Mandy Jeske

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sherif Ismail *Heidelberg University Biochemistry Center, Heidelberg University, Heidelberg 69120, Germany.ORCID 0000-0002-6063-1475
Jana Kubíková *Heidelberg University Biochemistry Center, Heidelberg University, Heidelberg 69120, Germany.ORCID 0009-0009-2248-4134
Maria Maichel *Heidelberg University Biochemistry Center, Heidelberg University, Heidelberg 69120, Germany.ORCID 0009-0005-0038-5785
Peter AndersenDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus C 8000, Denmark.ORCID 0000-0001-5815-4860
Mandy JeskeHeidelberg University Biochemistry Center, Heidelberg University, Heidelberg 69120, Germany.ORCID 0000-0002-1107-9442

Funding

Chica and Heinz Schaller Foundation (CHS-Stiftung) n.a.Danmarks Frie Forskningsfond (DFF) 9064-00056BDeutsche Forschungsgemeinschaft (DFG) JE-827/1-1Deutsche Forschungsgemeinschaft (DFG) JE-827/1-2Novo Nordisk Fonden (NNF) NNF18OC0030954
6 · The paper itself

Abstract

Nearly all biological processes depend on protein-protein interactions (PPIs). While various methods exist to study these interactions, investigating those that involve nuclear proteins, including structurally complex proteins containing long disordered regions, remains a significant challenge. Here, we developed nxReLo, a simple and fast cell culture-based colocalization assay, designed to identify and characterize interactions between nuclear proteins. PIWI-interacting RNAs (piRNAs) safeguard germline genome integrity, and the PPIs that facilitate piRNA production are therefore essential for animal reproduction, yet remain incompletely understood. We used nxReLo to investigate interactions between members of the

Indexed as

Drosophila ProteinsNuclear ProteinsRNA-Binding ProteinsAnimalsArgonaute ProteinsDrosophila melanogasterProtein BindingRNA, Small InterferingArgonaute ProteinsDrosophila ProteinsNuclear ProteinsRNA-Binding ProteinsRNA, Small Interferingcell-based interaction assayDrosophilanuclear proteinspiRNAprotein–protein interaction

Identifiers

PMID41296720
PMCPMC12685051

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.