Evidence map›Paper›PMID 41296081›Full record

ArticlePediatric surgery international2025

Ferrostatin-1 protects against necrotizing enterocolitis intestinal injury by inhibiting ferroptosis.

Chen-Yi Wang, Mehrsa Feizi, Bo Li, Carol Lee, Dorothy Lee, Jielin Yang, Ying Kang, Yu-Zuo Bai, Agostino Pierro

Abstract read
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Article in Pediatric surgery international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chen-Yi WangDepartment of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
Mehrsa FeiziTranslational Medicine Program, Division of General and Thoracic Surgery, Department of Translational Medicine, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Bo LiTranslational Medicine Program, Division of General and Thoracic Surgery, Department of Translational Medicine, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Carol LeeTranslational Medicine Program, Division of General and Thoracic Surgery, Department of Translational Medicine, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Dorothy LeeTranslational Medicine Program, Division of General and Thoracic Surgery, Department of Translational Medicine, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Jielin YangTranslational Medicine Program, Division of General and Thoracic Surgery, Department of Translational Medicine, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Ying KangTranslational Medicine Program, Division of General and Thoracic Surgery, Department of Translational Medicine, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
Yu-Zuo BaiDepartment of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
Agostino PierroTranslational Medicine Program, Division of General and Thoracic Surgery, Department of Translational Medicine, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada. agostino.pierro@sickkids.ca.

Funding

China Scholarship Council 202408210240CIHR 353857
6 · The paper itself

Abstract

purposeNecrotizing enterocolitis (NEC) is a severe neonatal disease marked by intestinal injury, and epithelial damage has been linked to ferroptosis. This study aimed to determine the protective effect of Ferrostatin-1 (Fer-1), a ferroptosis inhibitor, on NEC-associated intestinal injury.

methodsNEC was induced in mouse pups via formula feeding, hypoxia, and lipopolysaccharide exposure. Fer-1 (5 mg/kg) was administered intraperitoneally on postnatal days 6 and 8. Intestinal tissues were analyzed for morphological injury, epithelial proliferation (Ki67), ferroptosis markers (Gpx4 and Tfr1), and lipid peroxidation (4-HNE). Human NEC intestinal organoids derived from surgical samples were treated with Fer-1 (2 µM) for 48 h. Levels of ferrous ion (FerroOrange), lipid peroxide (BODIPY), and reactive oxygen species (DCFDA) were measured.

resultsFer-1 significantly reduced NEC-induced epithelial injury in mice, leading to improved intestinal morphology and increased epithelial proliferation, as indicated by elevated Ki67 expression. The protective effect was associated with reduced ferroptosis, demonstrated by upregulated Gpx4 expression and decreased levels of Tfr1 and 4-HNE. Similarly, in human NEC organoids, Fer-1 significantly reduced the accumulation of ferrous ions, lipid peroxides, and ROS.

conclusionFer-1 effectively protects against NEC-induced intestinal injury by inhibiting ferroptosis and reducing oxidative stress. These findings highlight its potential as a novel therapeutic strategy for managing intestinal damage in NEC.

Indexed as

CyclohexylaminesEnterocolitis, NecrotizingFerroptosisPhenylenediaminesAnimalsAnimals, NewbornDisease Models, AnimalHumansIntestinal MucosaLipid PeroxidationMiceMice, Inbred C57BLCyclohexylaminesferrostatin-1PhenylenediaminesFerroptosisFerrostatin-1Intestinal organoidsNecrotizing enterocolitis

Identifiers

PMID41296081

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.